Collaborative Research: EDGE FGT: Development of genetic and functional genomics resources for Ipomoea (morning glory)

合作研究：EDGE FGT：Ipomoea（牵牛花）遗传和功能基因组学资源的开发

• 批准号: 2220214
• 负责人: Regina Baucom
• 金额: $ 109.51万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2220214&HistoricalAwards=false
• 关键词: Collaborative; Research; EDGE; FGT; Development

Understanding how plants adapt to their environment has become more important than ever in a time of climate change. For many decades, morning glories have been the species of choice for studying fundamental questions in evolutionary biology, particularly for weedy morning glories, ornamentals, and the globally important food crop, sweetpotato. Such evolutionary studies can be aided through the application of modern molecular and genomic tools; yet, while morning glories are a captivating and economically important group, resources for linking genetics and variation in form are lacking. This project combines state-of-the-art approaches to discover how changes in DNA influence plant form and ecological function including responses to environmental change. The development of these approaches in model morning glory species will broaden the scope of current evolutionary investigations. Further, the dissemination of newly developed genetic resources and research protocols will assist the larger research community studying the molecular basis of variation in plant traits and adaptations. To reach a broad audience, including plant scientists and undergraduate students, YouTube videos will be developed as will methods designed for undergraduate laboratory courses in plant evolution, genetics, and genomics. Additionally, student artists will capture variation in morning glory traits from a garden display held at the Matthaei Botanical Garden, and these artistic renditions will be displayed at the Natural History Museum in Ann Arbor, Michigan, thus reaching the public via multiple avenues.Novel transformation techniques using multiple Agrobacterium strains and the development of mutant lines via the use of fast neutrons (FN) have expanded transformation capabilities to a wider group of plants and have likewise allowed for the relatively rapid development of random mutations for understanding gene function. This project will adapt these methods to morning glory species (Ipomoea) that have been used in ecological and evolutionary genetics applications (I. purpurea, I. hederacea, I. lacunosa), species that are close relatives of the important crop sweetpotato (I. trifida and I. lacunosa), or phylogenetically useful outgroups (I. obscura). Genetic mapping populations for I. lacunosa and I. purpurea will be generated, genotyped, and disseminated for use by scientists that seek to interrogate a number of evolutionary and applied questions. Gene expression profiles for these populations will likewise be generated. Education of the plant science community in transformation protocols and the distribution of resources are significant foci of this work. An Ipomoea Wiki will be developed to integrate and disseminate protocols, videos, and sequence data links to allow researchers and educators to reconstruct the process of genetic transformation and to easily locate project data. Sequences will be deposited to public databases as generated, following initial cleaning steps. This project will support 1) training opportunities for undergraduate researchers along with members of the plant science community including graduate students, postdoctoral researchers, and professors, 2) public displays and artistic renditions that capture the remarkable and memorable variation found within and between species belonging to the Ipomoea genus.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在气候变化时期，了解植物如何适应环境变得比以往任何时候都变得更加重要。数十年来，早晨的荣耀一直是研究进化生物学基本问题的选择，尤其是对于杂草凌晨，荣耀，观念和全球重要的粮食作物Sweetpotato。这种进化研究可以通过现代分子和基因组工具的应用得到帮助。然而，尽管早晨的荣耀是一个令人着迷且在经济上重要的群体，但缺乏将遗传学和形式变化联系起来的资源。该项目结合了最新的方法，以发现DNA的变化如何影响植物形式和生态功能，包括对环境变化的反应。这些方法在模型晨荣耀物种中的发展将扩大当前进化研究的范围。此外，新开发的遗传资源和研究方案的传播将有助于更大的研究社区研究植物特征和适应性变化的分子基础。为了吸引广泛的受众，包括植物科学家和本科生，将开发YouTube视频，以及为植物进化，遗传学和基因组学的本科实验室课程而设计的方法。此外，学生艺术家将在Matthaei植物园举行的花园展览中捕捉晨间荣耀特质的变化，这些艺术演绎将在密歇根州安阿伯的自然历史博物馆展出，从而通过多个Avenues.novel Transformation。使用多个农杆菌菌株和通过使用快速中子（FN）开发突变线的技术已扩展到更广泛的植物的转化能力，并且同样允许相对快速发展随机突变以了解基因功能。该项目将这些方法适应在生态和进化遗传学应用中使用的早晨荣耀物种（ipomoea）（I. Trifida和I. lacunosa）或系统发育有用的外组（I. subscura）。将为lacunosa和I. purpurea的基因映射种群生成，基因分型和传播，以供科学家使用，这些科学家试图询问许多进化和应用问题。这些人群的基因表达谱也将同样产生。植物科学界在转型方案中的教育和资源分配是这项工作的重要焦点。将开发一个iPomoea Wiki，以整合和传播协议，视频和序列数据链接，以允许研究人员和教育工作者重建遗传转化的过程并轻松找到项目数据。序列将按照初始清洁步骤作为生成的公共数据库存入。该项目将支持1）培训本科研究人员以及植物科学界的成员，包括研究生，博士后研究人员和教授，2）公开展示和艺术演绎，这些培训和艺术演绎捕获了属于属于的物种内部和属于的显着变化ipomoea属。该奖项反映了NSF的法定任务，并被认为是通过基金会的知识分子优点和更广泛影响的评论标准来评估值得支持的。