Collaborative Research: EDGE FGT: DEVELOPMENT OF FUNCTIONAL GENETIC TOOLS IN GECKOS

合作研究：EDGE FGT：壁虎功能遗传工具的开发

• 批准号: 10673453
• 负责人: Anthony B Gamble
• 金额: $ 37.29万
• 依托单位: MARQUETTE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10673453
• 关键词: Address; Biology; Clustered Regularly Interspaced Short Palindromic Repeats; Comparative Study; Development; Educational workshop; Genes; Genetic; Genome; Genotype; Human; Knowledge; Lizards; Methods; Modeling; Modification; Oocytes; Pathway interactions; Phenotype; Production; Reptiles; Research; Research Personnel; Snakes; Techniques; Training; Work; functional genomics; gene function; genome editing; genomic tools; human disease; improved; tool

Functional genomic tools, such as CRISPR/cas genome editing, are critical for investigating the biology of genes and have transformed our understanding of genotype-to-phenotype relationships. However, the limited number of species with genome modification methods presents a major bottleneck in implementing comprehensive, comparative studies of gene function. For instance, squamates (lizard and snakes) include more than 11,000 described species and comprise 40% of all amniote species. Yet basic gene editing methods are only available in a single species, the brown anole lizard (Anolis sagrei). Here, we propose to: 1) establish CRISPR/cas gene editing in a second lizard group, the geckos; 2) develop advanced gene editing methods for the introduction of precise sequence substitutions, insertions, and deletions in lizards (in both Anolis and geckos); and 3) disseminate lizard gene editing techniques through training materials and workshops and the production of genome-edited lizards for researchers. The development of gene editing tools in geckos will increase the number of squamate models available for functional genomic work and facilitate the generalization of gene function studies across squamates and amniotes. Furthermore, growing the functional genomic toolkit in lizards to include more precise gene editing methods will expand the scope of questions that can be addressed in this phenotypically diverse group.

功能基因组工具，例如 CRISPR/cas 基因组编辑，对于研究 基因生物学并改变了我们对基因型与表型关系的理解。 然而，具有基因组修饰方法的物种数量有限，这是一个主要问题 实施全面的基因功能比较研究的瓶颈。例如， 有鳞动物（蜥蜴和蛇）包括 11,000 多种已描述的物种，占所有物种的 40% 羊膜动物种类。然而基本的基因编辑方法仅适用于单一物种，即棕色 安乐蜥蜴（Anolis sagrei）。在此，我们建议：1）秒建立CRISPR/cas基因编辑 蜥蜴群，壁虎； 2）开发先进的基因编辑方法，引入精准基因 蜥蜴（安乐蜥和壁虎）中的序列替换、插入和删除；和 3) 通过培训材料和讲习班传播蜥蜴基因编辑技术 为研究人员生产基因组编辑的蜥蜴。基因编辑工具的发展 壁虎将增加可用于功能基因组工作的有鳞动物模型的数量 促进有鳞动物和羊膜动物基因功能研究的推广。此外， 在蜥蜴中开发功能基因组工具包以包括更精确的基因编辑方法将 扩大这个表型多样化群体中可以解决的问题范围。