RAPID: Next phase serological testing for SARS-CoV-2 for biocultural research

RAPID：用于生物文化研究的 SARS-CoV-2 下一阶段血清学检测

基本信息

批准号：
2035114
负责人：
Thomas McDade
金额：
$ 19.92万
依托单位：
Northwestern University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-15 至 2022-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2035114&HistoricalAwards=false
关键词：
RAPID Next phase serological testing

项目摘要

This RAPID project will develop methods and infrastructure for SARS-CoV-2 antibody and immunity testing that can be operationalized to examine a wide range of social and biocultural processes as they relate to COVID-19 exposure and outcomes. Results from a large community-based sample will be used to inform estimates of the seroprevalence of infection in order to illuminate the geographic spread of the virus, identify subgroups of individuals more susceptible to infection, and investigate the development of immunity following exposure. A minimally-invasive approach to antibody testing will facilitate application in community-based settings and provide information on the predictors of viral transmission that can be used to mitigate future outbreaks. The project will also contribute to the infrastructure of science through training opportunities for graduate students and postdoctoral researchers, and includes public outreach activities on the role of antibody testing for SARS-CoV-2. During the COVID 19 pandemic, strategic testing in community-based settings is critical for understanding the true level of infection, tracking the virus, and for preventing transmission. Serological testing for antibodies against the virus is an important tool for identifying individuals who have been exposed to SARS-CoV-2, but current clinic-based testing approaches require serum samples collected via venipuncture, which is difficult to implement when people are being asked to stay at home. This project combines the convenience of blood collection in the home with the analytic rigor that can be applied in the laboratory by using a serological test for SARS-CoV-2 IgG antibodies that requires only a single drop of blood, collected on filter paper following a simple finger stick. The investigators will deploy a web-based, “no-contact” research platform to investigate the origins of differences in COVID-19 infection rates across neighborhoods in Chicago. Following recruitment, participants navigate to a home page with their smart phone or computer where consent is obtained and a survey is administered electronically. A kit is mailed for the collection of a finger stick dried blood spot sample, which is returned to the lab and analyzed for IgG antibodies against the receptor binding domain of SARS-CoV-2. Test results will be combined with survey responses and neighborhood-based administrative data to investigate the individual-, household-, and community-level predictors of exposure. The second aim is to develop and validate a surrogate virus neutralization protocol for use with dried blood spot samples. This protocol addresses an important limitation of current antibody tests which detect the presence of binding antibodies, but cannot quantify the presence of the neutralizing antibodies that actually prevent the virus from entering host tissues upon re-infection. The protocol will then be applied to samples from the first aim to investigate the factors that predict the development of immunity to SARS-CoV-2.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该 RAPID 项目将开发 SARS-CoV-2 抗体和免疫测试的方法和基础设施，可用于检查与 COVID-19 暴露和结果相关的广泛社会和生物文化过程。样本将用于估计抗体的血清流行率，以阐明病毒的地理传播情况，识别更容易受到感染的个体亚组，并调查暴露后免疫力的发展。一种微创检测方法将有助于促进病毒的传播。应用在该项目还将通过为研究生和博士后研究人员提供培训机会，并包括有关该角色的公共宣传活动，为科学基础设施做出贡献。在 COVID 19 大流行期间，在社区环境中进行战略性检测对于了解感染的真实水平、追踪病毒以及预防病毒抗体的传播至关重要。识别曾接触过的个人的重要工具SARS-CoV-2，但目前基于临床的检测方法需要通过静脉穿刺采集血清样本，当人们被要求呆在家里时，这很难实施。该项目将家庭采血的便利性与分析的严谨性结合起来。可以在实验室中应用 SARS-CoV-2 IgG 抗体的血清学测试，只需通过简单的手指刺破将一滴血液收集在滤纸上。研究人员将部署一个基于网络的“不”测试。 -接触”研究招募后，参与者使用智能手机或计算机导航到主页，并通过电子方式进行调查。收集指尖干燥的血斑样本，将其送回实验室并分析针对 SARS-CoV-2 受体结合域的 IgG 抗体。测试结果将与调查答复和基于社区的管理数据相结合，以调查该情况。个人、家庭和社区层面的预测因素第二个目标是开发和验证用于干血斑样本的替代病毒中和方案，该方案解决了当前抗体测试的一个重要局限性，该测试检测结合的存在，但无法量化中和抗体的存在。实际上，该协议将应用于样本，其首要目的是防止预测 SARS-CoV-2 免疫力发展的因素。该奖项反映了 NSF 的法定使命，并已获得批准。认为值得支持使用基金会的智力价值和更广泛的影响审查标准进行评估。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Comparison of IgG and neutralizing antibody responses after one or two doses of COVID-19 mRNA vaccine in previously infected and uninfected individuals.

DOI：
10.1016/j.eclinm.2021.101018
发表时间：
2021-08
期刊：
EClinicalMedicine
影响因子：
15.1
作者：
Demonbreun AR;Sancilio A;Velez MP;Ryan DT;Saber R;Vaught LA;Reiser NL;Hsieh RR;D'Aquila RT;Mustanski B;McNally EM;McDade TW
通讯作者：
McDade TW

Geographic disparities in COVID-19 case rates are not reflected in seropositivity rates using a neighborhood survey in Chicago.

DOI：
10.1016/j.annepidem.2021.10.006
发表时间：
2022-03
期刊：
Annals of epidemiology
影响因子：
5.6
作者：
Mustanski B;Saber R;Ryan DT;Benbow N;Madkins K;Hayford C;Newcomb ME;Schrock JM;Vaught LA;Reiser NL;Velez MP;Hsieh RR;Demonbreun AR;D'Aquila R;McNally EM;McDade TW
通讯作者：
McDade TW

Patterns and persistence of SARS-CoV-2 IgG antibodies in Chicago to monitor COVID-19 exposure

DOI：
10.1172/jci.insight.146148
发表时间：
2021-05-10
期刊：
JCI INSIGHT
影响因子：
8
作者：
Demonbreun, Alexis R.;McDade, Thomas W.;McNally, Elizabeth M.
通讯作者：
McNally, Elizabeth M.

Cohabitation With a Known Coronavirus Disease 2019 Case Is Associated With Greater Antibody Concentration and Symptom Severity in a Community-Based Sample of Seropositive Adults.

DOI：
10.1093/ofid/ofab244
发表时间：
2021-07
期刊：
Open forum infectious diseases
影响因子：
4.2
作者：
Schrock JM;Ryan DT;Saber R;Benbow N;Vaught LA;Reiser N;Velez MP;Hsieh R;Newcomb M;Demonbreun AR;Mustanski B;McNally EM;D'Aquila R;McDade TW
通讯作者：
McDade TW

Durability of antibody response to vaccination and surrogate neutralization of emerging variants based on SARS-CoV-2 exposure history.