Plant-microbe strategies for utilization of mineral-associated P sources

利用矿物相关磷源的植物微生物策略

• 批准号: 240950800
• 负责人: Professor Dr. Bruno Glaser
• 金额: --
• 依托单位: Professur für Bodenkunde und Bodenschutz
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/240950800?language=en
• 关键词: Plant; microbe; strategies; utilization; mineral

Depleted resources in easy accessible apatite as well as the sorption of inorganic and organic phosphorus (P) to secondary minerals are the main proposed aspects for P limitation of old weathered forest ecosystems. In spite of that indications exist that sorption of P is not irreversible and that life communities are capable to recycle mineral-associated P. But the underlying processes and controlling factors are still poorly known. In this project we will test the hypothesis that particularly in P-limited ecosystems plant-microbe communities can utilize mineral-bound P. We assume that a higher P use efficiency from secondary minerals, especially of sorbed orthophosphate in comparison to P monoesters, is due to an enhanced investment of photosynthates into weathering-active mycorrhiza and bacteria, with such a strategy being realized for both ectomycorrhized plants as well as for plants living in symbiosis with arbuscular fungi. Therefore, mesocosm experiments with soils varying in P supply and amended with additional P sources, i.e. ferrihydrite with adsorbed orthophosphate and P-monoesters, will be run under Fagus sylvatica (ectomycorrhiza) und Acer pseudoplatanus (arbuscular mycorrhiza). 13CO2 isotope labeling and substance-specific 13C analysis in phospholipid fatty acids and low-molecular weight organic acids as well as enzyme activity measurements will inform about the investment of plants into microbial communites as well as weathering agents as a function of soil P availability and P types present. Further, 18O-labeling of phosphate in combination with NanoSIMS application will be used to directly reveal the utilization of mineral-bound P. In a field exposure experiment, P adsorption complexes will be buried and incubated for one year to verify the mesocosms results. Phosphorus losses will be assessed by X-ray fluorescence and photoelectron spectroscopy and the involved microbial communities will be described based on quantitative real-time PCR and 454 pyrosequencing. The obtained data will clarify whether the recycling of P from secondary minerals is a relevant process and which strategies of life are realized.

易于获得的磷灰石中的资源以及无机和有机磷（P）的吸附到二次矿物质中是P限制旧风化森林生态系统的主要方面。尽管存在这种迹象表明，P的吸附不是不可逆转的，并且生命社区能够回收与矿物质相关的P。但是，基本的过程和控制因素仍然鲜为人知。 In this project we will test the hypothesis that particularly in P-limited ecosystems plant-microbe communities can utilize mineral-bound P. We assume that a higher P use efficiency from secondary minerals, especially of sorbed orthophosphate in comparison to P monoesters, is due to an enhanced investment of photosynthates into weathering-active mycorrhiza and bacteria, with such a strategy being realized for both牙齿化植物以及与羊膜真菌共生的植物。因此，在P供应中使用土壤变化的土壤实验，并用其他P来源（即带有吸附的正磷酸盐和P-Monoesters）进行修改，将在Fagus sylvatica（Ectomycorrhiza）下运行。 13CO2同位素标记和物质特异性13C分析在磷脂脂肪酸和低分子量有机酸以及酶活性的测量结果中，将为植物投资于微生物通讯中的投资，以及伴有土壤P的可用性和P类型的函数。此外，将磷酸盐与纳米菌素施用结合使用18o标记，将用于直接揭示矿物结合P的利用。在野外暴露实验中，P吸附配合物将埋葬并孵育一年，以验证中cosms的结果。磷损失将通过X射线荧光和光电子光谱法评估，并将基于定量的实时PCR和454焦磷酸测序来描述所涉及的微生物群落。获得的数据将阐明次级矿物质的P回收是否是一个相关过程，并且实现了哪些生活策略。