BEE: Spatio-temporal factors shaping symbiotic networks: a case study with cyanolichens

BEE：塑造共生网络的时空因素：氰基地衣的案例研究

基本信息

批准号：
1929994
负责人：
Francois Lutzoni
金额：
$ 41.31万
依托单位：
Duke University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-01 至 2025-08-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1929994&HistoricalAwards=false
关键词：
BEE Spatio temporal factors shaping

项目摘要

Symbiotic interactions, including parasitism, commensalism and mutualism, form complex networks that are common in nature. Ultimately, a more complete understanding of life can be accomplished only through the study of all symbiotically interacting organisms. This study aims to understand the processes that shape those networks using cyanolichens, which are iconic symbioses consisting mainly of mutually beneficial fungi and cyanobacteria. Questions that will be addressed by this project include: Does the abundance of various free-living cyanobacteria in nature dictate the main lichen partner pairs (one fungus, one cyanobacterium) who shape lichen communities? Do broadly distributed cyanobacteria who associate with many fungal species (generalists) do so mostly with closely related fungal species? What are the biomolecules that determine which cyanobacteria interact with which fungal partners? How do the rules of association for lichen partners differ when compared to other symbiotic systems? This project includes training and educational components for graduate and undergraduate students, the dissemination of new methods for innovative research on symbiotic systems, outreach activities, and the development of a new advising model to help underrepresented students interested in science and engineering reach their academic goals. The main focus of this study is to determine how mutualistic networks are shaped by evolution and environmental gradients at different spatial scales. To reach this goal, a fully integrated approach combining evolutionary biology and ecology will be implemented. Recent studies of the lichen model genus Peltigera and its Nostoc symbionts have revealed: (i) that Peltigera species are mostly specialized on generalist Nostoc phylogroups, (ii) that the interactions and the high level of specialization by the fungus are phylogenetically conserved, and (iii) that bioclimatic factors, at an intra-biome scale, are more limiting than cyanobiont availability for explaining geographic distributions of Peltigera species. This study will capitalize on the large-scale, systematic, and multi-taxon, monitoring program of the Alberta Biodiversity Monitoring Institute (ABMI). This project represents a unique opportunity to test three important drivers of symbiotic network assembly: (1) Spatial scales and gradients - Are interactions well predicted by environmental biotic and abiotic properties, and/or spatial overlap due to stochastic dispersal? (2) Phylogenetic history - Do Nostoc symbionts show phylogenetic conservatism in their selection of Peltigera partners, as shown by Peltigera towards their Nostoc partners? (3) Specific recognition molecules - Are Nostoc genes differentially expressed in lichen thalli vs a free-living state, and do they explain the observed specificity between symbiotic partners in nature?This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

共生相互作用，包括寄生虫，共同主义和互助，形成了自然界中常见的复杂网络。最终，只有通过研究所有共生相互作用的生物，才能实现对生命的更完整的理解。这项研究旨在了解使用氰基化塑造这些网络的过程，这些过程主要由互惠互利真菌和蓝细菌组成。该项目将解决的问题包括：自然界中各种自由生活的蓝细菌的丰富性决定了塑造地衣社区的主要地衣伴侣对（一种真菌，一个蓝细菌）？与许多真菌物种（通才）相关的广泛分布的蓝细菌主要与密切相关的真菌物种这样做？哪些生物分子决定了哪种蓝细菌与哪个真菌伴侣相互作用？与其他共生系统相比，地衣伙伴的结合规则有何不同？该项目包括针对研究生和本科生的培训和教育组成部分，传播有关共生系统的创新研究，外展活动的创新方法，以及开发新的建议模型，以帮助对科学和工程感兴趣的代表性不足的学生达到他们的学术目标。这项研究的主要重点是确定在不同空间尺度下的进化和环境梯度如何塑造共同的网络。为了实现这一目标，将实施一种结合进化生物学和生态学的完全整合的方法。最近对peligera属及其nostoc symbionts的研究表明：（i）peltigera物种主要是专门用于通才的nostoc thyologroups，（ii）真菌的相互作用和高水平的专业化是在生物源代源性的范围内，并且（III）更高的是cen cel，并且（iii）是cen cel的含量，并且比例更高，并且是cen cel的范围。 Peltigera物种的地理分布。这项研究将利用艾伯塔省生物多样性监测研究所（ABMI）的大规模，系统和多核心监测计划。该项目代表了测试共生网络组装的三个重要驱动因素的独特机会：（1）空间尺度和梯度 - 是否通过环境生物和非生物特性和/或由于随机分散引起的空间重叠来很好地预测相互作用？（2）系统发育史 - 如Peltigera对Nostoc伴侣的选择，Nostoc共生体在选择Peltigera伴侣时是否显示出系统发育保守主义？（3）特定的识别分子 - 在地衣thalli与自由生活状态的Nostoc基因是否差异表达，它们是否解释了自然界中共生伙伴之间观察到的特异性？该奖项反映了NSF的法定任务，并通过评估该基金会的知识分子优点和广泛的影响来评估NSF的法定任务，并被视为值得的支持。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Ancient Rapid Radiation Explains Most Conflicts Among Gene Trees and Well-Supported Phylogenomic Trees of Nostocalean Cyanobacteria

古代快速辐射解释了念珠藻蓝藻基因树和良好支持的系统发育树之间的大多数冲突

DOI：
10.1093/sysbio/syad008
发表时间：
2023
期刊：
Systematic Biology
影响因子：
6.5
作者：
Pardo-De la Hoz, Carlos J.;Magain, Nicolas;Piatkowski, Bryan;Cornet, Luc;Dal Forno, Manuela;Carbone, Ignazio;Miadlikowska, Jolanta;Lutzoni, François
通讯作者：
Lutzoni, François

High species richness in the lichen genus Peltigera (Ascomycota, Lecanoromycetes): 34 species in the dolichorhizoid and scabrosoid clades of section Polydactylon, including 24 new to science

Peltigera 地衣属（子囊菌门、Lecanoromycetes）物种丰富度高：多指菌科的长根菌和粗根菌分支中有 34 种，其中包括 24 种新科学物种

DOI：
10.3767/persoonia.2023.51.01
发表时间：
2023
期刊：
Persoonia - Molecular Phylogeny and Evolution of Fungi
影响因子：
0
作者：
Magain, N.;Miadlikowska, J.;Goffinet, B.;Goward, T.;Pardo-De la Hoz, C.J.;Jüriado, I.;Simon, A.;Mercado-Díaz, J.A.;Barlow, T.;Moncada, B.
通讯作者：
Moncada, B.

Towards a nomenclatural clarification of the Peltigera ponojensis/monticola clade including metagenomic sequencing of type material and the introduction of P. globulata Miadl. & Magain sp. nov.