RoL: FELS: EAGER: Mining for ancient toolkits: Emergence of planar structures across kingdoms

RoL：FELS：渴望：古代工具包的开采：各个王国平面结构的出现

• 批准号: 1838291
• 负责人: Aleksandar Popadic
• 金额: $ 30万
• 依托单位: Wayne State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-11-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1838291&HistoricalAwards=false
• 关键词: RoL; FELS; EAGER; Mining; ancient

Both Animal and Plant Kingdoms have evolved flat lateral (planar) appendages, for example, wings and leaves. Despite the lack of obvious functional similarity, planar structures in animals and plants may result from the merging of dorsal and ventral tissues, each of which is defined by different gene regulatory networks. This study will carry out a series of comparative gene expression analyses across homologous and analogous organs within Classes and across Kingdoms in order to determine whether there are shared, and perhaps universal, gene expression networks regulating the development of planar appendages. The data and insights may enable the development of new fundamental concepts in biology. This project will also enhance student learning in undergraduate and graduate courses and provide opportunities for participating undergraduate students to develop their critical thinking. Due to its location and university mission, Wayne State has a deep commitment to encouraging underrepresented and first-generation college students to explore careers in science and to develop a deep appreciation of science with respect to our national welfare. Studies of multiple organisms demonstrate that although dorsal/ventral (adaxial/abaxial) gene regulatory networks (GRNs) are necessary for planar structures, they are not sufficient. This fact suggests that there must be additional, presently undefined, emergent GRNs that are required for planar development. The experimental design is based on comparative transcriptome analyses between planar and non-planar homologous organs and planar and non-planar non-homologous, analogous organs in selected lineages of angiosperms and arthropods. These comparative analyses will seek to detect regulatory modules at different levels and help identify shared and perhaps universal toolboxes governing the development of planar structures. Data from RNAseq libraries will be mapped to de novo assemblies to quantify expression, and to determine gene functional identities. Expression counts of genes will be comprehensively analyzed to identify functional co-expression gene networks. Finally, detailed biological patterning across Classes and Kingdoms will be performed through analysis of protein domains within networks to detect deep overlapping regulatory modules.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

动物王国和植物王国都在横向（平面）附属物中演变出来，例如翅膀和叶子。尽管缺乏明显的功能相似性，但动物和植物中的平面结构可能是由于背侧和腹侧组织的合并而导致的，每种组织都由不同的基因调节网络定义。 这项研究将在班级和跨王国内进行一系列跨同源和类似器官进行一系列比较基因表达分析，以确定是否有共享，也许是普遍的基因表达网络来调节平面附件的发展。数据和见解可以使生物学中新的基本概念的发展。该项目还将增强学生在本科和研究生课程中的学习，并为参与本科生发展批判性思维的机会提供机会。由于其位置和大学任务，韦恩州立大学致力于鼓励人数不足和第一代大学生探索科学职业，并在我们的国家福利方面对科学产生深厚的欣赏。对多种生物的研究表明，尽管背侧/腹侧（术/赤字）基因调节网络（GRN）对于平面结构是必需的，但它们还不够。这一事实表明，平面开发所需的其他目前必须有其他，目前未定义的，紧急的GRN。实验设计基于平面和非平面同源器官以及平面和非平面非同源的，类似的器官之间的比较转录组分析。 这些比较分析将寻求检测不同级别的监管模块，并有助于确定有关平面结构开发的共享或普遍的工具箱。 来自RNASEQ库的数据将映射到从头组件中以量化表达，并确定基因功能身份。基因的表达计数将进行全面分析，以识别功能共表达基因网络。 最后，将通过分析网络内的蛋白质领域来检测深层重叠的监管模块来进行详细的生物学模式。该奖项反映了NSF的法定任务，并被认为值得通过基金会的知识分子优点和更广泛的影响来评估通过评估来进行评估。