Collaborative Research: Precise and Dexterous Single-Particle Manipulation Using Non-uniform AC Magnetic Fields

合作研究：利用非均匀交流磁场进行精确灵巧的单粒子操纵

• 批准号: 1808307
• 负责人: Rui Qiao
• 金额: $ 21.44万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-06-15 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1808307&HistoricalAwards=false
• 关键词: Collaborative; Research; Precise; Dexterous; Single

High-precision manipulation of single micro-particles such as biological cells and colloids in the liquid environment is a critical process in applications such as single-cell analysis. A myriad of methods has been developed to achieve such manipulation. However, few of them can simultaneously meet all the requirements in practical applications, e.g., high precision, robustness, ability to move particles along arbitrary paths, low cost, and good biocompatibility. Recently the principal investigators discovered a new kind of particle manipulation method, i.e., using non-uniform alternating magnetic fields to actuate an anisotropic magnetic cluster and further applying the actuated cluster to manipulate nonmagnetic particles. Unlike other magnetic manipulation methods, this method requires only low-frequency, weak magnetic fields, and two orders of magnitude less power to achieve the same transitional speed, and the entire setup is extremely cost-effective. However, the fundamental mechanisms underlying this method are not clear and the parameters to precisely control the cluster motion are unknown. This project seeks to resolve this challenge and thus to create a precise, dexterous, low-cost, and biocompatible method for manipulating single particles. The project can potentially enable better single-cell analysis and make such analysis more accessible to research and educational communities, thereby creating great scientific and societal impact. The project includes education programs involving undergraduate students with diverse ethnical backgrounds and regional K-12 students. Discoveries from the project will be disseminated to technical as well as general audiences.The objective of this project is to understand, prefect, and apply the newly discovered magnetic particle actuation method through two specific aims: (1) to understand the actuation of single magnetic particles using non-uniform alternating magnetic fields; (2) to investigate nonmagnetic particle manipulation through the actuation of single magnetic particles. These aims will be achieved by integrating magnetic particle fabrication, experimental characterization of particulate dynamics in liquids, and multiphysics simulations. These interdisciplinary activities will benefit from the synergistic collaboration of the two research teams at University of Georgia and Virginia Tech, which have a fruitful history of collaboration and demonstrated expertise in material synthesis, instrumentation, and experimental and computational studies of particle transport in low-Reynolds number flows. The results from this project will provide both the theoretical basis and practical guidelines for the effective design of systems to manipulate single particles and cells. This project will also create new knowledge on the dynamics of magnetic particles in liquid environments and hydrodynamic actuation of particles in low-Reynolds number flows, thereby simultaneously contributing to the fields of magnetic actuation, fluid dynamics, particle assembly, and biotechnology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在液体环境中，高精度操纵单微粒（例如生物细胞和胶体）是单细胞分析等应用中的关键过程。已经开发了无数方法来实现这种操纵。但是，很少有人能同时满足实际应用中的所有要求，例如高精度，鲁棒性，沿任意路径，低成本和良好的生物相容性移动颗粒的能力。最近，主要研究人员发现了一种新型的粒子操纵方法，即使用不均匀的交替磁场来启动各向异性磁簇，并进一步应用该驱动的群集来操纵非磁性颗粒。与其他磁性操纵方法不同，此方法仅需要低频，弱磁场和两个数量级以实现相同的过渡速度的功率降低，并且整个设置的成本效益极高。但是，该方法依据的基本机制尚不清楚，精确控制群集运动的参数尚不清楚。该项目旨在解决这一挑战，从而创建一种处理单个颗粒的精确，灵巧，低成本和生物相容性的方法。该项目可以潜在地实现更好的单细胞分析，并使研究和教育社区更容易获得此类分析，从而产生巨大的科学和社会影响。该项目包括涉及具有不同种族背景和区域K-12学生的本科生的教育计划。从项目中发现的发现将被传播到技术和一般受众。该项目的目的是通过两个特定目的来理解，完善并应用新发现的磁性粒子驱动方法：（1）使用非均匀的交替磁场来理解单个磁性颗粒的驱动； （2）通过单个磁颗粒的致动来研究非磁性颗粒操作。这些目标将通过整合磁性颗粒制造，液体中颗粒动力学的实验表征以及多物理模拟来实现。这些跨学科活动将受益于佐治亚大学和弗吉尼亚大学的两个研究团队的协同合作，这些研究团队具有合作的富有成果的历史，并在材料综合，仪器，仪器以及低雷诺数量流中粒子运输的实验和计算研究方面具有专业知识。该项目的结果将提供理论基础和实用指南，以有效设计操纵单个颗粒和细胞的系统。该项目还将创建有关液体环境中磁性颗粒动力学的新知识，以及低雷诺数数量流中颗粒的流体动力学致动，从而同时为磁性致动，流体动力学，粒子组装和生物技术学领域做出了贡献。这些奖项通过NSF的法规及其构成的依据，及其在范围内的范围及其授予的构成均具有良好的影响。 标准。

项目成果

Magnetic Actuation of Surface Walkers: The Effects of Confinement and Inertia

• DOI: 10.1021/acs.langmuir.9b03487
• 发表时间: 2020-06-30
• 期刊: LANGMUIR
• 影响因子: 3.9
• 作者: Fang, Wen-Zhen;Ham, Seokgyun;Tao, Wen-Quan
• 通讯作者: Tao, Wen-Quan

Manipulation of Single Cells Using a Ferromagnetic Nanorod Cluster Actuated by Weak AC Magnetic Fields

使用弱交流磁场驱动的铁磁纳米棒簇操纵单细胞

• DOI: 10.1002/adbi.201800246
• 发表时间: 2018
• 期刊: Advanced Biosystems
• 影响因子: 4.1
• 作者: Zhu, Lu;Huang, Weijie;Yang, Fengchang;Yin, Lei;Liang, Shenxuan;Zhao, Wujun;Mao, Leidong;Yu, Xiaozhong;Qiao, Rui;Zhao, Yiping
• 通讯作者: Zhao, Yiping