Combining Algal and Plant Photosynthesis (CAPP2): Application to Extend the NSF/BBSRC PSIL Collaboration

结合藻类和植物光合作用 (CAPP2)：扩展 NSF/BBSRC PSIL 合作的应用

• 批准号: 1737710
• 负责人: Martin Jonikas
• 金额: $ 27.67万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-01-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1737710&HistoricalAwards=false
• 关键词: Combining; Algal; Plant; Photosynthesis; CAPP2

Productivity in many crops is limited by CO2 availability and water use. The CAPP project aims to overcome those limitations by transferring a performance-enhancing Carbon Concentrating Mechanism (CCM) from green algae to higher plants. This project will yield significant advances in our understanding in the function of CCMs, which remain mysterious even though nearly all algae use them to assimilate CO2. If successful, the project will be a key step towards significant increases in crop productivity. More generally, this project represents one of the first attempts at transplanting a complex system from one organism to another, and thus will present many opportunities for learning general principles that will be useful as mankind increasingly attempts to engineer biology.CAPP2 will focus on understanding the pyrenoid, an organelle at the core of the algal CCM, and re-creating its key features in the C3 plant Arabidopsis. Structure, assembly and regulation of the pyrenoid will be studied in the green alga Chlamydomonas, using mutants, light and electron microscopy, and biochemistry. Components will be introduced into Arabidopsis, and the impact on productivity will be modeled and measured. Additionally, a Förster Resonance Energy Transfer (FRET) nanosensor will be developed to measure concentrations of bicarbonate with sub-cellular resolution in vivo; the sensor will be applied to characterize the CCM in both Chlamydomonas and Arabidopsis.

许多作物的生产力受到二氧化碳可用性和用水量的限制，CAPP 项目旨在通过将提高性能的碳浓缩机制 (CCM) 从绿藻转移到高等植物来克服这些限制。尽管几乎所有藻类都利用 CCM 来吸收二氧化碳，但 CCM 的功能仍然很神秘。如果成功，该项目将成为显着提高作物生产力的关键一步。更广泛地说，该项目是首批尝试之一。能够将复杂的系统从一种生物体移植到另一种生物体，因此将提供许多学习一般原理的机会，随着人类越来越多地尝试设计生物学，这些原理将很有用。CAPP2 将重点了解核糖体，这是藻类 CCM 核心的细胞器，并利用突变体、光学和电子显微镜，在绿藻衣藻中研究蛋白核的结构、组装和调节，并在 C3 植物拟南芥中重新创建其关键特征。此外，还将开发福斯特共振能量转移（FRET）纳米传感器，以测量体内亚细胞分辨率的碳酸氢盐浓度；可用于表征衣藻和拟南芥中的 CCM。