Scalable Manufacturing of Nanostructured Bioassemblies for Low-Cost Portable Biosensors

用于低成本便携式生物传感器的纳米结构生物组件的可扩展制造

• 批准号: 1561491
• 负责人: Emanuela Andreescu
• 金额: $ 31.57万
• 依托单位: Clarkson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1561491&HistoricalAwards=false
• 关键词: Scalable; Manufacturing; Nanostructured; Bioassemblies; Low

There is a growing demand for developing manufacturing processes that can reliably and reproducibly generate nanostructured materials with functional bioactive properties at low cost and in large quantities. These technologies are needed in a wide range of applications, especially in medical diagnostics and in environmental and food monitoring. Examples include responsive materials for wearable biosensing devices, flexible bioelectronics, functional contact lenses, smart screens and intelligent packaging. For example, bioactive nanostructures that have the appropriate detection sensitivity and selectivity are particularly important for the development of low cost devices for home and point-of-care diagnosis. This research will develop a process for large scale manufacturing of functional bioactive nanostructures on flexible and inexpensive substrates such as paper and plastic, using three-dimensional (3D) printing. This award will enable the development of a new class of biosensing devices that are easy to use, portable and inexpensive. Research outcomes will be disseminated through journals and conference presentations, including hands-on demonstrations of the sensing capabilities of the nanomanufactured devices, curriculum enhancement and professional development workshops for high school teachers, collaboration with industry, and providing entrepreneurial training for students.This project will develop scalable fabrication methods for nanobioactive materials with defined optical and electronic properties and biological functionality. The overall goal is to gain fundamental understanding of the formation mechanism of hybrid bioactive nanostructures by printing and to use this knowledge to develop scalable production methods for low cost diagnostic applications. The research team will focus on the engineering design of eco-friendly printing inks of characteristic viscosity and composition that maintain functional nanoscale properties and bioactivity. These nanostructures will integrate biorecognition, signal amplification and detection capabilities and will function as all-in-one biosensing devices. Fabrication steps and detection schemes will be developed to facilitate automatic printing of the entire sensing unit and enable reagentless operation. The approach can be expanded to the synthesis of other hybrid nanostructures and devices with tailored functionality. The method will open the way for the in situ assembly of hybrid bioactive nanostructures using a low cost, versatile and controllable manufacturing process.

人们对开发制造工艺的需求不断增长，这些制造工艺能够以低成本和大批量可靠、可重复地生产具有功能性生物活性特性的纳米结构材料。这些技术有广泛的应用，特别是在医疗诊断以及环境和食品监测方面。例子包括用于可穿戴生物传感设备、柔性生物电子学、功能性隐形眼镜、智能屏幕和智能包装的响应材料。例如，具有适当检测灵敏度和选择性的生物活性纳米结构对于开发用于家庭和护理点诊断的低成本设备尤其重要。这项研究将开发一种利用三维（3D）打印在柔性且廉价的基材（例如纸张和塑料）上大规模制造功能性生物活性纳米结构的工艺。该奖项将有助于开发易于使用、便携且廉价的新型生物传感设备。研究成果将通过期刊和会议演讲传播，包括纳米制造设备传感能力的实践演示、针对高中教师的课程强化和专业发展研讨会、与业界的合作以及为学生提供创业培训。该项目将开发具有明确光学和电子特性以及生物功能的纳米生物活性材料的可扩展制造方法。总体目标是通过打印获得对混合生物活性纳米结构形成机制的基本了解，并利用这些知识开发用于低成本诊断应用的可扩展生产方法。研究团队将专注于环保印刷油墨的工程设计，其特性粘度和成分可保持功能性纳米级特性和生物活性。这些纳米结构将集成生物识别、信号放大和检测功能，并将作为一体化生物传感设备。将开发制造步骤和检测方案，以促进整个传感单元的自动打印并实现无试剂操作。该方法可以扩展到其他混合纳米结构和具有定制功能的设备的合成。该方法将为使用低成本、多功能且可控的制造工艺原位组装混合生物活性纳米结构开辟道路。