Collaborative Research: Biomagnetic Glasses: Preparation, Characterization and Biosensor Applications

合作研究：生物磁性玻璃：制备、表征和生物传感器应用

• 批准号: 0804506
• 负责人: Emanuela Andreescu
• 金额: --
• 依托单位: Clarkson University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0804506&HistoricalAwards=false
• 关键词: Collaborative; Research; Biomagnetic; Glasses; Preparation

Lead ID: DMR/BMAT(7623) 0804464 Lead PI: Stanciu, Lia ORG: Purdue Non-Lead ID: DMR/BMAT(7623) 0804506 Non-Lead PI: Andreescu, Emanuela ORG: Clarkson Title: COLLABORATIVE RESEARCH: Biomagnetic Glasses: Preparation, Characterization, and Biosensor Applications INTELLECTUAL MERIT: The goal of this proposal is to create a generic approach for the fabrication of biofunctional materials with magnetic capabilities, so called "biomagnetic glasses." These will be used in the design of highly stable, magnetically separable enzyme-based systems. The project focuses on creating hybrid composite materials containing enzymes, magnetic nanoparticles, and silica glasses and on the development of generic strategies that will be used to prepare these materials. Three enzymes have been selected as model examples: horseradish peroxidase (HRP), acetylcholinesterase (AChE), and glucose-6-phosphate dehydrogenase (G6PDH). As an example of proposed applications, the AChE based biomagnetic glasses will be suitable for use as the sensing component in the development of a cheap and autonomously operating bioanalytical device for detecting pesticides in food products, agricultural fields and other security situations. It is proposed that the magnetic properties of the sensor materials will permit them to be localized with respect to the potential analytes using permanent magnets. The specific objectives of the project are: (1) synthesize and characterize silica-iron oxide magnetic nanoparticles with controlled microstructure and properties. (Purdue); (2) encapsulate model enzymes (HRP, AChE, G6PDH) within the silica magnetic nanocomposites and monitor the biocatalytic activity and stability (Purdue); (3) use screen printed electrodes to exploit the biomagnetic glasses in biosensors (Clarkson); (4) build calibration plots of the biosensors upon application of various concentrations of target analytes (e.g. hydrogen peroxide, pesticides, glucose-6-phosphate) (Clarkson). BROADER IMPACTS: Bio-based sensor technology that is sensitive and selective across a broad dynamic range remains in great demand for analysis of chemical species in biotech process streams, in the environment, and in the security area. This proposal addresses, in particular, the issue of useful lifetime of enzyme-based biosensors. The project provides an excellent platform for multidisciplinary training of graduate and undergraduate students. Both PIs encourage undergraduate participation; funding for support of undergraduates is included in the Clarkson budget. The Purdue-based PI plans to incorporate materials on biosensors in courses that she teaches in the two Schools of which she is a member. Both PIs will engage in outreach to local public schools. At Clarkson these relationships are established and on-going, while the PI at Purdue is developing the necessary relationships with the school system. She is also active in the Purdue Women in Engineering program and has been successful in recruiting female students into her research group. Both PIs demonstrate a sensitivity toward broadening participation among underrepresented minority students. The PIs are connected with ongoing minority recruitment programs on their respective campuses.

Lead ID: DMR/BMAT(7623) 0804464 Lead PI: Stanciu, Lia ORG: Purdue Non-Lead ID: DMR/BMAT(7623) 0804506 Non-Lead PI: Andreescu, Emanuela ORG: Clarkson Title: COLLABORATIVE RESEARCH: Biomagnetic Glasses: Preparation, Characterization, and Biosensor Applications INTELLECTUAL优点：该提案的目的是为具有磁性能力（所谓的“生物磁玻璃”）制造生物功能的材料来创建一种通用方法。这些将用于设计高度稳定的基于磁性酶的系统。该项目着重于创建含有酶，磁性纳米颗粒和二氧化硅玻璃的混合复合材料，以及将用于制备这些材料的通用策略的发展。已经选择了三种酶作为模型示例：辣根过氧化物酶（HRP），乙酰胆碱酯酶（ACHE）和葡萄糖-6-磷酸脱氢酶（G6PDH）。作为提议的应用的一个例子，基于ACHE的生物磁玻璃将适合用作开发廉价且自主运行的生物分析装置的感应组件，用于检测食品，农业领域和其他安全情况中的农药。有人提出，传感器材料的磁性特性将允许使用永久磁铁对潜在分析物进行定位。该项目的特定目标是：（1）合成并表征具有控制的微结构和特性的二氧化硅氧化物磁性纳米颗粒。 （普渡大学）; （2）将模型酶（HRP，ACHE，G6PDH）封装在二氧化硅磁性纳米复合材料内，并监测生物催化活性和稳定性（Purdue）； （3）使用丝网印刷电极来利用生物传感器中的生物磁玻璃（Clarkson）； （4）在应用各种目标分析物（例如，过氧化氢，农药，6-磷酸葡萄糖）（Clarkson）时，建立生物传感器的校准图。更广泛的影响：在广泛的动态范围内具有敏感且有选择性的基于生物的传感器技术，对在生物技术过程，环境和安全区域中对化学物种的分析的需求仍然很大。该建议特别是针对基于酶的生物传感器的有用寿命问题。该项目为研究生和本科生的多学科培训提供了一个绝佳的平台。两种PI都鼓励本科参与；克拉克森预算中包括支持本科生的资金。总部位于普渡大学的PI计划将有关生物传感器的材料纳入她在她的两所学校中教授的课程中。这两个PI都将与当地公立学校进行宣传。在克拉克森（Clarkson）建立了这些关系和持续的关系，而普渡大学的PI正在发展与学校系统的必要关系。她还活跃于普渡大学工程计划中，并成功地招募了女学生加入她的研究小组。这两个PI都表现出对扩大人数不足的少数族裔学生的参与的敏感性。 PI与各自校园中持续的少数族裔招聘计划有关。