Collaborative Research: Biomagnetic Glasses: Preparation, Characterization and Biosensor Applications

合作研究：生物磁性玻璃：制备、表征和生物传感器应用

• 批准号: 0804506
• 负责人: Emanuela Andreescu
• 金额: --
• 依托单位: Clarkson University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0804506&HistoricalAwards=false
• 关键词: Collaborative; Research; Biomagnetic; Glasses; Preparation

Lead ID: DMR/BMAT(7623) 0804464 Lead PI: Stanciu, Lia ORG: Purdue Non-Lead ID: DMR/BMAT(7623) 0804506 Non-Lead PI: Andreescu, Emanuela ORG: Clarkson Title: COLLABORATIVE RESEARCH: Biomagnetic Glasses: Preparation, Characterization, and Biosensor Applications INTELLECTUAL MERIT: The goal of this proposal is to create a generic approach for the fabrication of biofunctional materials with magnetic capabilities, so called "biomagnetic glasses." These will be used in the design of highly stable, magnetically separable enzyme-based systems. The project focuses on creating hybrid composite materials containing enzymes, magnetic nanoparticles, and silica glasses and on the development of generic strategies that will be used to prepare these materials. Three enzymes have been selected as model examples: horseradish peroxidase (HRP), acetylcholinesterase (AChE), and glucose-6-phosphate dehydrogenase (G6PDH). As an example of proposed applications, the AChE based biomagnetic glasses will be suitable for use as the sensing component in the development of a cheap and autonomously operating bioanalytical device for detecting pesticides in food products, agricultural fields and other security situations. It is proposed that the magnetic properties of the sensor materials will permit them to be localized with respect to the potential analytes using permanent magnets. The specific objectives of the project are: (1) synthesize and characterize silica-iron oxide magnetic nanoparticles with controlled microstructure and properties. (Purdue); (2) encapsulate model enzymes (HRP, AChE, G6PDH) within the silica magnetic nanocomposites and monitor the biocatalytic activity and stability (Purdue); (3) use screen printed electrodes to exploit the biomagnetic glasses in biosensors (Clarkson); (4) build calibration plots of the biosensors upon application of various concentrations of target analytes (e.g. hydrogen peroxide, pesticides, glucose-6-phosphate) (Clarkson). BROADER IMPACTS: Bio-based sensor technology that is sensitive and selective across a broad dynamic range remains in great demand for analysis of chemical species in biotech process streams, in the environment, and in the security area. This proposal addresses, in particular, the issue of useful lifetime of enzyme-based biosensors. The project provides an excellent platform for multidisciplinary training of graduate and undergraduate students. Both PIs encourage undergraduate participation; funding for support of undergraduates is included in the Clarkson budget. The Purdue-based PI plans to incorporate materials on biosensors in courses that she teaches in the two Schools of which she is a member. Both PIs will engage in outreach to local public schools. At Clarkson these relationships are established and on-going, while the PI at Purdue is developing the necessary relationships with the school system. She is also active in the Purdue Women in Engineering program and has been successful in recruiting female students into her research group. Both PIs demonstrate a sensitivity toward broadening participation among underrepresented minority students. The PIs are connected with ongoing minority recruitment programs on their respective campuses.

铅 ID：DMR/BMAT(7623) 0804464 铅 PI：Stanciu、Lia ORG：普渡大学 非铅 ID：DMR/BMAT(7623) 0804506 非铅 PI：Andreescu、Emanuela ORG：Clarkson 标题：协作研究：生物磁性眼镜：制备、表征和生物传感器应用 知识优点：该提案的目标是创建一种通用方法来制造具有磁性的生物功能材料，即所谓的“生物磁玻璃”。这些将用于设计高度稳定、磁性可分离的酶系统。该项目的重点是创建含有酶、磁性纳米颗粒和二氧化硅玻璃的混合复合材料，并开发用于制备这些材料的通用策略。选择三种酶作为模型实例：辣根过氧化物酶（HRP）、乙酰胆碱酯酶（AChE）和葡萄糖-6-磷酸脱氢酶（G6PDH）。作为拟议应用的一个例子，基于乙酰胆碱酯酶的生物磁性玻璃将适合用作传感组件，用于开发廉价且自主操作的生物分析设备，用于检测食品、农田和其他安全情况中的农药。据建议，传感器材料的磁性将允许它们使用永磁体相对于潜在的分析物进行定位。该项目的具体目标是：（1）合成并表征具有受控微观结构和性能的二氧化硅-氧化铁磁性纳米颗粒。 （普渡大学）； (2) 将模型酶（HRP、AChE、G6PDH）封装在二氧化硅磁性纳米复合材料中并监测生物催化活性和稳定性（Purdue）； （3）利用丝网印刷电极开发生物传感器中的生物磁性玻璃（Clarkson）； (4) 在应用不同浓度的目标分析物（例如过氧化氢、杀虫剂、6-磷酸葡萄糖）时建立生物传感器的校准图（Clarkson）。更广泛的影响：生物技术工艺流程、环境和安全领域的化学物质分析仍然需要在广泛的动态范围内具有灵敏性和选择性的生物传感器技术。该提案特别解决了基于酶的生物传感器的使用寿命问题。该项目为研究生和本科生的多学科培养提供了良好的平台。两位 PI 都鼓励本科生参与；支持本科生的资金包含在克拉克森预算中。这位来自普渡大学的 PI 计划将生物传感器材料纳入她在她所在的两所学校教授的课程中。两位 PI 都将参与当地公立学校的外展活动。在克拉克森，这些关系已经建立并持续存在，而普渡大学的 PI 正在与学校系统建立必要的关系。她还积极参与普渡大学女性工程项目，并成功招募女学生加入她的研究小组。两位 PI 都表现出对扩大代表性不足的少数族裔学生的参与的敏感性。 PI 与各自校园正在进行的少数族裔招聘计划有关。