DISSERTATION RESEARCH: Consequences of sympatry and allopatry for variation in reproductive genes of Drosophila pseudoobscura

论文研究：同源性和异源性对果蝇生殖基因变异的影响

基本信息

批准号：
1500911
负责人：
Leonie Moyle
金额：
$ 2.01万
依托单位：
Indiana University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2015
资助国家：
美国
起止时间：
2015-06-01 至 2016-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1500911&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH Consequences sympatry allopatry

项目摘要

Many factors influence mate choice in both plants and animals, including choosing a mate that will provide the best quality offspring while avoiding individuals of different species. The integration of these factors into a single choice implies that traits involved in mate selection within species could be the same traits that cause species to diverge from one another. Recently, the investigators used genetically modified Drosophila melanogaster to show that the same genes that control within species mate choice are also involved in species discrimination. This project is testing this association in a natural system, to understand how different populations accumulate changes in genes that control mate choice and species discrimination. These data will provide insight into the molecular mechanisms underlying natural variation in mate choice and the formation of reproductively isolated populations, which are important factors in understanding how new species arise over evolutionary time. The proposed research will examine DNA sequence and gene expression variation in reproductive genes in natural populations of the fruit fly Drosophila pseudoobscura, using high depth nucleotide sequencing. The experiment contrasts genetic changes that have occurred in populations that co-occur with closely related species, and thus must discriminate against them during mate choice, with changes in populations that are not exposed to other species. Analyses will assess genetic variation across all reproductive genes, as well as specifically in genes known to affect gamete competition. These variants will be associated with phenotypic data on mate choice within and between species, to differentiate genes that are important to mate choice, to species discrimination, and to both reproductive behaviors.

许多因素都会影响动植物中的伴侣选择，包括选择一个将提供最佳质量后代的伴侣，同时避免不同物种的个体。将这些因素整合到一个选择中意味着，物种中伴侣选择涉及的特征可能是使物种彼此不同的特征。最近，研究人员使用了基因改良的果蝇Melanogaster，以表明在物种选择中控制物种中的相同基因也参与了物种歧视。该项目正在自然系统中测试这种关联，以了解不同人群如何积累控制伴侣选择和物种歧视的基因的变化。这些数据将洞悉伴侣选择中自然变化的分子机制和生殖孤立的种群的形成，这是了解新物种在进化时期如何出现的重要因素。拟议的研究将使用高深度核苷酸测序来检查果蝇果蝇pseudoobscura的自然种群中生殖基因中的DNA序列和基因表达变异。该实验与与密切相关的物种同时发生的种群中发生的遗传变化进行了对比，因此必须在伴侣选择期间对其进行区分，而人群中未暴露于其他物种的人群的变化。分析将评估所有生殖基因的遗传变异，以及专门针对会影响配子竞争的基因。这些变体将与物种内部和物种之间的伴侣选择的表型数据相关，以区分对选择，物种歧视以及两种生殖行为至关重要的基因。