Comparative Genomics of Hybrid Incompatibility in Lycopersicon

番茄杂种不亲和性的比较基因组学

• 批准号: 0444946
• 负责人: Leonie Moyle
• 金额: --
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-15 至 2005-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0444946&HistoricalAwards=false
• 关键词: Comparative; Genomics; Hybrid; Incompatibility; Lycopersicon

This research focuses on Lycopersicon - the species group that includes the domesticated tomato - to identify the genetic basis of hybrid pollen and seed sterility, two of the strongest reproductive isolating barriers among species in the Solanaceae, the tomato family. Using comparative genomic trait mapping between five different Lycopersicon species, in combination with classical genetics and phenotyping, the research will: 1) identify the number, individual effect, and location within the genome, of genetic regions that cause pollen and seed sterility among species; 2) determine the phenotypic consequences of interactions among these different genetic regions; and, 3) develop the groundwork to identify individual genes directly responsible for interspecific reproductive barriers among Lycopersicon species.Reproductive isolation barriers are a substantial obstacle to agricultural exploitation of wild germplasm and are fundamental to the origin of biodiversity. Nonetheless, very little is known about the genes responsible for reproductive isolation, especially among plant species. This research will provide detailed insight into the genetic basis of hybrid sterility among multiple species in an economically important plant group. Research products will include genomic data and resources that may increase the accessibility of agriculturally useful traits found in wild species, and thereby aid the development of new crop resources. Due to their close relationship, this work may also help in identifying genes responsible for hybrid sterility in other economically important plants, including potato, eggplant, and pepper. The research will also provide a diverse training environment for undergraduates, graduate students, and post-doctoral researchers, in plant breeding, genomics, and evolutionary biology.

这项研究的重点是Lycoperson（包括驯化番茄）的物种群，以识别杂化花粉和种子不育的遗传基础，这是溶酶科中物种中最强的生殖隔离屏障之一，番茄家族。研究将利用五种不同的lycopersicon物种之间的比较基因组特征映射，结合经典的遗传学和表型，研究将：1）确定基因组内的数量，个体效应和位置，这些遗传区域在物种之间导致花粉和种子不育的遗传区域； 2）确定这些不同遗传区域之间相互作用的表型后果； 3）开发基础，以确定直接负责番茄红素物种中种间生殖屏障的单个基因。生殖隔离屏障是农业剥削野生种质的巨大障碍，并且对生物多样性的起源至关重要。尽管如此，对于负责生殖隔离的基因，尤其是在植物物种中，知之甚少。这项研究将提供对经济重要植物群中多种物种中混合不育的遗传基础的详细见解。研究产品将包括基因组数据和资源，这些数据和资源可能会增加野生物种中农业有用特征的可及性，从而有助于开发新的作物资源。由于它们的密切关系，这项工作也可能有助于确定负责其他经济重要植物（包括马铃薯，茄子和胡椒）中混合无菌性的基因。这项研究还将为植物育种，基因组学和进化生物学的大学生，研究生和博士后研究人员提供多种培训环境。