BREAD EAGER: Analysis of Bovine Epididymal and Chicken SST Sperm to Determine Proteins Involved in Sperm Preservation

BREAD EAGER：分析牛附睾和鸡 SST 精子以确定参与精子保存的蛋白质

• 批准号: 1446886
• 负责人: George Perry
• 金额: $ 30万
• 依托单位: South Dakota State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1446886&HistoricalAwards=false
• 关键词: BREAD; EAGER; Analysis; Bovine; Epididymal

Over 92 species of reptiles, amphibians, birds, and mammals have developed the ability to store ejaculated sperm in the female reproductive tract for extended periods of time (days to years) with no decrease in its ability to fertilize the ovum. However, in most mammals sperm can be maintained for up to 15 days in the cauda epididymis, but only live for approximately 24 hours after ejaculation. The long term goal of this research is to determine the mechanisms by which sperm can be stored long term in the female reproductive tract of chickens and in the epididymis of the bull, and to utilize the knowledge to develop a cold-chain-free system to allow artificial insemination of domestic farms animals, especially in developing countries that do not have the infrastructure for cryopreservation. A thorough understanding of the proteins that create the microenvironment of the epididymis and chicken semen storage tubules will advance our understanding of how these proteins may enhance membrane integrity to improve long term storage. Leveraging the new knowledge of the epididymal environment should enable cold-free sperm storage for extended periods, and may have other applications for somatic cell preservation. Studies supported by this award will utilize 2D LC-MS/MS to compare protein profiles of bovine epididymal sperm and ejaculated sperm to the protein profiles of chicken sperm stored in the female reproductive tract, and determine the mitochondrial activity in each group of sperm. To determine if increased longevity is accomplished through decreased activity. Ejaculated bull sperm will then be washed and cultured in media containing epididymal or chicken proteins at the pH of epididymal sperm to determine changes in sperm kinetics. The PI will recruit one Master's student to be an integral part of this proposal, and will continue to recruit undergraduate students and international interns for research training and direct involvement in the research efforts. Results from the study will be published in peer-reviewed journals and disseminated through scientific conferences.

超过92种爬行动物，两栖动物，鸟类和哺乳动物已经发展出了在女性生殖道中储存射精的精子的能力，长时间（天到几年）没有降低其卵子受精的能力。但是，在大多数哺乳动物中，精子可以在cauda附睾中保持长达15天，但仅在射精后大约生存24小时。这项研究的长期目标是确定可以长期存储精子在女性鸡生殖道和公牛的繁殖道中的机制，并利用知识来开发无冷链的系统来允许家庭农场的人工启发，尤其是在不具有冰冻分析的发展中国家的人造农场动物中。对蛋白质的透彻理解，这些蛋白质创造了附睾和鸡肉精液储存小管的微环境将提高我们对这些蛋白质如何增强膜完整性以改善长期存储的理解。 利用附子环境的新知识应使无冷精子存储长时间，并且可能还具有其他用于躯体细胞保存的应用。该奖项支持的研究将利用2D LC-MS/MS比较牛子附子精子的蛋白质谱，并射精的精子与雌性生殖道中储存的鸡精子的蛋白质谱，并确定每组精子中的线粒体活性。确定是否通过减少活动来实现寿命的增加。然后将射精的牛精子洗涤和培养在含有附睾精子pH的附睾或鸡蛋白的培养基中，以确定精子动力学的变化。 PI将招募一位硕士学生成为该提案不可或缺的一部分，并将继续招募本科生和国际实习生进行研究培训，并直接参与研究工作。 该研究的结果将在同行评审的期刊上发表，并通过科学会议传播。