EAGER: Metabolomics Analysis of Archival Marine Invertebrates

EAGER：档案海洋无脊椎动物的代谢组学分析

• 批准号: 2341344
• 负责人: Bill Baker
• 金额: $ 28.73万
• 依托单位: University of South Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2341344&HistoricalAwards=false
• 关键词: EAGER; Metabolomics; Analysis; Archival; Marine

Non-technical project descriptionMuseums of natural history, such as the Smithsonian National Museum of Natural History (NMNH) in Washington, D.C., are repositories for, among other things, biological specimens. Specimens stored at the NMNH were obtained over many decades and across the globe, resulting in what is currently a treasure trove of biological and chemical information. Chemical compounds (metabolites) found in the tissues of, for example, marine invertebrates, can record the organism’s response to a changing environment. This project seeks to establish a strategy for analyzing these compounds in Antarctic marine invertebrates held in the NMNH. These organisms are especially valuable for studies of their metabolites as such information will contribute to our understanding of the history of the polar environment and how organisms are able to adapt to extreme habitats. Further, studies of these rare and difficult to obtain metabolites have broad impacts in biotechnology and human health.Technical description of the projectThis project seeks to develop a workflow for the analysis of metabolites in archival marine invertebrate specimens held in the Smithsonian National Museum of Natural History (NMNH). Recent advances in mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy, both instrumental as well as analysis platforms, enable the detection and annotation of chemical structures in these otherwise difficult to obtain metabolites. In particular, NMR strategy (Pure Shift NMR) will be implemented to increase sensitivity toward these sample-limited analytes. Further, the workflow will be applied in an analysis of storage methods used by the NMNH with the aim of understanding how best to preserve specimens for future metabolomics analyses. With an optimized workflow established, additional applications to inform our understanding of adaptation and (cryptic) speciation in the extreme habitats found in Antarctica are possible.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

非技术项目描述自然历史的穆斯，例如华盛顿特区的史密森尼国家自然历史博物馆（NMNH），是生物标本的存储库。在NMNH中存储的标本是在全球数十年和全球范围内获得的，这导致了目前的生物和化学信息的宝库。在海洋无脊椎动物的组织中发现的化合物（代谢产物）可以记录生物体对环境变化的反应。该项目旨在制定一项策略，以分析NMNH中持有的南极海洋无脊椎动物中这些化合物。这些生物对于研究其代谢产物特别有价值，因为这样的信息将有助于我们对极地环境的历史以及生物如何适应极端栖息地的理解。此外，对这些罕见且难以获得代谢产物的研究对生物技术和人类健康产生了广泛的影响。该项目项目的技术描述旨在开发一个工作流程，以分析代谢物毫无疑问的海洋无脊椎动物标本，该标本在史密森尼国家自然史（NMNH）（NMNH）。质谱（MS）和核磁共振（NMR）光谱的最新进展，包括工具性和分析平台，可以在这些原本难以获得代谢物的这些化学结构中检测和注释。特别是，将实施NMR策略（纯移位NMR），以提高对这些样品有限分析物的敏感性。此外，工作流程将应用于NMNH使用的存储方法的分析，目的是了解如何最好地保存标​​本以进行未来的代谢组学分析。通过建立了优化的工作流程，可以通过基金会的智力优点和更广泛的审查标准来评估NSF的法定任务，以告知我们对南极洲极端栖息地的理解和（隐秘的）规格的理解。