MRI: Acquisition of a Tandem MS for Plant Cellular & Subcellular Metabolic Studies

MRI：获取用于植物细胞的串联 MS

• 批准号: 1427621
• 负责人: Bradley Evans
• 金额: $ 45.74万
• 依托单位: Donald Danforth Plant Science Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2018-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1427621&HistoricalAwards=false
• 关键词: MRI; Acquisition; Tandem; MS; Plant

An award is made to the Donald Danforth Plant Science Center to purchase a tandem mass spectrometer for plant cellular and subcellular metabolic studies. The investment in mass spectrometry technology and method development at the Danforth Center benefits integrated studies of plant metabolism and provides guiding protocols for the greater scientific community. The projects supported by this new instrumentation aim to increase productivity of food and biofuel crops through a detailed understanding of how plants turn energy provided by the sun to biomass and how plants sense and respond to their environment and attack by pathogens. As part of the development of new technologies, PI Bradley Evans and Co-PI Doug Allen will perform a special study with young investigators from Harris-Stowe State University to probe the new instrument's capability to analyze difficult samples. This will add to the educational outreach taking place at the Danforth Center. Currently, the training of tomorrow's scientists occurs through an active NSF-REU program, recruitment of high school students that work in Danforth Center laboratories for classroom credit, participation in a regional program for high school students - STARS (Students and Teachers As Research Scientists), workshops for teachers and an interactive middle- and high school mentoring program that connects our scientists with nearby schools. These programs are essential to core Danforth Center mission goals to strengthen the plant science community.The long term goal is to improve plant productivity that: i) proficiently feeds a growing world population, ii) provides sustainable feed-stock alternatives to petroleum and iii) supplies useful bioactive compounds; all designed to improve quality of life. Advanced tandem mass spectrometry instrumentation is needed to investigate metabolites present at very low and dynamic levels and segregated to specific cells and organelles. A major impediment to understanding biochemical function in plants is our incomplete characterization of metabolism. Plant metabolism is a very dynamic process and operates in tiny compartments and low amounts of material. Thus it is the fine architecture and dynamic metabolic events throughout plant life that define function, fitness, agricultural yield and allow plants to respond to changing environments and stresses. These activities are, however, not quantitatively understood and incomplete metabolic characterization prevents elucidation of biochemical pathways and gene and protein functions, thereby precluding successful applications in biotechnology. Sensitive mass spectrometers that are capable of analyzing minute concentrations have the potential to transform understanding in many aspects of plant function. Research efforts on NSF-funded and other projects at the Donald Danforth Plant Science Center and surrounding community are advancing science through methods development on scantly present metabolites and will be greatly extended by this technology.

向唐纳德·丹佛斯植物科学中心颁发了奖励，以购买植物细胞和亚细胞代谢研究的双人质谱仪。丹佛斯中心的质谱技术和方法开发的投资使植物代谢的综合研究受益，并为更大的科学界提供指导方案。这种新仪器支持的项目旨在通过详细了解太阳提供的植物如何将生物量的能量转化为生物量，以及植物如何对环境和病原体的攻击来提高食物和生物燃料作物的生产率。作为新技术开发的一部分，Pi Bradley Evans和Co-Pi Doug Allen将与Harris-Stowe State University的年轻研究人员进行一项特别研究，以探究新工具分析困难样本的能力。这将增加在丹佛斯中心举行的教育外展。目前，对明天科学家的培训是通过活跃的NSF-REU计划进行的，在Danforth Center实验室工作的高中生招募了课堂学分，参与了高中生的区域课程 - 明星（学生和老师作为研究科学家），教师的工作坊以及互动的中学和高中培训计划，与我们的科学家联系，与我们的科学家联系。这些计划对于加强植物科学界的核心丹佛特中心任务目标至关重要。长期目标是提高植物生产力：i）熟练地养活了不断增长的世界人口，ii）为石油和iii提供可持续的进食替代品）提供了有用的生物活性化合物；所有这些都是为了改善生活质量。需要先进的串联质谱仪器来研究存在非常低和动态水平的代谢物，并隔离到特定的细胞和细胞器中。理解植物生化功能的主要障碍是我们对代谢的不完全表征。植物代谢是一个非常动态的过程，在微小的隔间和材料量少。因此，整个植物生命中的精细结构和动态代谢事件定义了功能，健身，农业产量，并使植物能够对不断变化的环境和压力做出反应。但是，这些活性尚未定量理解和不完全的代谢表征阻止生化途径，基因和蛋白质功能，从而排除了在生物技术中的成功应用。能够分析微小浓度的敏感质谱仪具有改变植物功能许多方面的理解的潜力。 Donald Danforth植物科学中心和周边社区对NSF资助和其他项目的研究工作正在通过可靠地呈现代谢物的方法开发来推进科学，这项技术将大大扩展。