MRI: Acquisition of an Orbitrap Lumos Mass Spectrometer for Plant Proteomics and Lipidomics

MRI：购买 Orbitrap Lumos 质谱仪用于植物蛋白质组学和脂质组学

• 批准号: 1827534
• 负责人: Bradley Evans
• 金额: $ 95.35万
• 依托单位: Donald Danforth Plant Science Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-10-01 至 2022-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1827534&HistoricalAwards=false
• 关键词: MRI; Acquisition; Orbitrap; Lumos; Mass

An award is made to the Donald Danforth Plant Science Center in Saint Louis, Missouri to purchase a high resolution/accurate mass Orbitrap mass spectrometer for plant proteomics and lipidomics studies. The investment in mass spectrometry technology and method development at the Danforth Center benefits integrated studies of plant development and metabolism and provides guiding protocols for the greater scientific community. The projects supported by this new instrumentation aim to increase productivity of food and biofuel crops through a detailed understanding of how plants turn energy provided by the sun to biomass and how plants sense and coordinate their physiology in response to seasonal change using their internal biological timepiece known as the circadian clock. As part of the development of new technologies, the investigators will perform a special study with undergraduates from Harris-Stowe State University and other nearby undergraduate institutions' students to mine the large data stream anticipated to come from the new instrument for biological treasure. The students will get firsthand experience with the contemporary proteomics techniques that may open the door to further bioanalytical studies in their careers. This will add to the educational outreach taking place at the Danforth Center. Currently, the training of tomorrow's scientists occurs through an active NSF-REU program, recruitment of high school students that work in Danforth Center laboratories for classroom credit, participation in a regional program for high school students - STARS (Students and Teachers As Research Scientists), workshops for teachers and an interactive middle- and high school mentoring program that connects our scientists with nearby schools. These programs are essential to core Danforth Center mission goals to strengthen the plant science community.The long-term goal is to improve plant productivity that: i) proficiently feeds a growing world population, ii) provides sustainable feed-stock alternatives to petroleum and iii) supplies useful bioactive compounds; all designed to improve quality of life. Advanced mass spectrometry instrumentation is needed perform complex stable isotope labeling experiments (an alternative to radioactive isotope labeling) and for characterizing post-translational modifications on proteins that are present a very low level in cells. A major impediment to understanding biochemical function in plants is our incomplete characterization of metabolism and proteins, the cellular machines that it. The dynamism of metabolite and protein levels, including their post-translational modifications (PTMs), are the result of linked networks that establish plant phenotype. Measurement of these biomolecules is critical to assessing plant health and response to changing environments; however, there are hundreds of thousands of metabolites found in the plant kingdom and organisms have more than a million of proteoforms that vary in abundance by many orders of magnitude and that change dynamically with time. Research efforts on NSF-funded and other projects at the Donald Danforth Plant Science Center and surrounding community are advancing science through methods development on scantly low abundance and highly dynamic proteins and metabolites and will be greatly extended by this technology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

向密苏里州圣路易斯的Donald Danforth植物科学中心颁发了奖励，以购买用于植物蛋白质组学和脂质组学研究的高分辨率/准确质量轨道质谱仪。丹佛斯中心质谱技术和方法开发的投资使植物开发和代谢的综合研究受益，并为更大的科学界提供指导方案。这种新仪器支持的项目旨在通过详细了解太阳提供的植物如何将生物量提供的能量转化为生物量，以及植物如何使用其内部生物钟表被称为昼夜节律时钟来提高食物和生物燃料作物的生产率。 作为新技术开发的一部分，研究人员将与哈里斯·斯托州立大学和附近其他本科机构的学生的本科生一起进行一项特别研究，以挖掘预计将来自新工具的生物宝藏的大型数据流。学生将获得当代蛋白质组学技术的第一手经验，这些技术可能为进一步的生物分析研究打开了他们的职业生物分析。 这将增加在丹佛斯中心举行的教育外展。目前，对明天科学家的培训是通过活跃的NSF-REU计划进行的，在Danforth Center实验室工作的高中生招募了课堂学分，参与了高中生的区域课程 - 明星（学生和老师作为研究科学家），教师的工作坊以及互动的中学和高中培训计划，与我们的科学家联系，与我们的科学家联系。这些计划对于加强植物科学界的核心丹佛特中心任务目标至关重要。长期目标是提高植物生产力：i）熟练地养活了不断增长的世界人口，ii）为石油和iii提供可持续的进食替代品）提供了有用的生物活性化合物；所有这些都是为了改善生活质量。需要进行高级质谱仪器进行复杂的稳定同位素标记实验（用于放射性同位素标记的替代方案），以表征对细胞水平非常低的蛋白质翻译后修饰。理解植物生化功能的主要障碍是我们对代谢和蛋白质的不完整表征，即它的细胞机。代谢产物和蛋白质水平的动力学（包括其翻译后修饰（PTM））是建立植物表型的链接网络的结果。这些生物分子的测量对于评估植物健康和对不断变化的环境的反应至关重要；但是，在植物王国中发现了数十万个代谢产物，生物具有超过一百万个蛋白质成型，这些蛋白基础在许多数量级上的丰富程度各不相同，并且随着时间的推移动态变化。 Donald Danforth植物科学中心和周围社区对NSF资助和其他项目的研究工作正在通过开发科学的发展，以低估的丰度和高度动态的蛋白质和代谢产物来发展，这项技术将大大扩展。该奖项反映了NSF的法定任务，并通过评估基金会的智力效果和广泛的评估来表现出值得的支持，并具有值得的支持。