IDBR: Type A: Collaborative research: High-speed AFM imaging of dynamics on biopolymers through non-raster scanning

IDBR：A 型：合作研究：通过非光栅扫描对生物聚合物动力学进行高速 AFM 成像

• 批准号: 1352729
• 负责人: Sean Andersson
• 金额: $ 46.11万
• 依托单位: Trustees of Boston University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-15 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1352729&HistoricalAwards=false
• 关键词: IDBR; Type; Collaborative; research; speed

This award by the Instrument Development for Biological Research (IDBR) program in the Division of Biological Infrastructure (BIO Directorate) is co-funded by the Particulate and Multiphase Processes (PMP) program in the Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET, Engineering Directorate).Non Technical AbstractThe primary aim of this project is to create a novel high-speed atomic force microscope (AFM) imaging system with frame rates of on the order of 100 frames/second. While the creation of this instrument may have a significant impact on a broad range of application areas, the primary target is the study of biomolecular processes. The extremely fast frame rate and long range will allow for the direct visualization of dynamic process that previously could be studied at best only through indirect means. The interdisciplinary nature of the project provides excellent opportunities for both graduate and undergraduate student training as well as outreach to middle- and high-school age students through summer programs on nano- and biotechnology.Technical AbstractThe speed gains of the instrument will be achieved in two ways. The first way is to replace the standard raster-scan of AFM with a feedback algorithm that steers the tip to stay in the region of interest. This will reduce imaging time by reducing the amount of scanning to be done, with the tradeoff that the sample to be imaged must have an underlying string-like structure such as is found in biopolymers. The second way is to create dual-stage scanning systems that will allow the non-raster scanning to be performed at ultra-fast speeds. A prototype system will be built and demonstrated on a variety of standard samples before being deployed to study the dynamics of the molecular motor myosin V. The instrument and underlying techniques will be disseminated through journal publication and conference publication and through collaboration with an existing AFM company to integrate the techniques in their next generation devices.

该奖项在生物学基础设施划分（BIO董事会）中获得的生物研究仪器开发计划（IDBR）计划由颗粒和多相过程（PMP）程序（PMP）计划（PMP）共同资助。 100帧/秒的顺序。尽管该仪器的创建可能会对广泛的应用领域产生重大影响，但主要目标是研究生物分子过程。极快的帧速率和远距离范围将允许直接可视化动态过程，而动态过程以前只能通过间接手段才能最多可以研究。该项目的跨学科性质为研究生和本科生培训以及通过有关纳米和生物技术的夏季计划向中学和高中生学生提供了极好的机会。技术摘要该乐器的速度提高将以两种方式实现。第一种方法是用一种反馈算法替换AFM的标准栅格扫描，该算法引导小费留在感兴趣的地区。这将通过减少要进行的扫描量来减少成像时间，而要进行的样品必须具有基础类似弦的结构，例如在生物聚合物中发现。第二种方法是创建双阶段扫描系统，该系统将允许以超快速速度执行非速度扫描。在部署以研究分子运动肌球蛋白V的动态之前，将在各种标准样本上建立和证明原型系统。仪器和基础技术将通过期刊出版物和会议出版物以及与现有的AFM公司的合作来传播，以将其整合到下一代式动任何股票中。