CAREER: Karrikin and strigolactone signaling mechanisms in Arabidopsis

职业：拟南芥中的 Karrikin 和独脚金内酯信号传导机制

• 批准号: 1350561
• 负责人: David Nelson
• 金额: $ 93.5万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-15 至 2017-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1350561&HistoricalAwards=false
• 关键词: CAREER; Karrikin; strigolactone; signaling; mechanisms

DAVID C NELSONPROPOSAL IOS-1350561CAREER: Karrikin and strigolactone signaling mechanisms in ArabidopsisKarrikins are a recently discovered class of compounds found in smoke that activate germination of many plant species after fire. Strigolactones comprise a family of plant hormones that regulate shoot branching, root architecture, and cambial growth. Strigolactones also trigger germination of the highly destructive parasitic weeds, broomrape and witchweed, and promote symbiotic associations with arbuscular mycorrhizal fungi. Understanding the molecular mechanisms by which karrikins and strigolactones control plant growth may enable a new wave of innovation in crop development and weed control.Plants recognize both karrikins and strigolactones through a genetic pathway that requires the F-box protein MAX2. F-box proteins have central roles in several plant hormone signaling systems, and act by targeting specific protein substrates for degradation. The targets of MAX2 have long remained elusive and as such represent a critical roadblock to understanding the mechanism of karrikin and strigolactone signaling. A genetic screen has now led to the identification of a candidate target, SUPPRESSOR OF MAX2 1 (SMAX1), which regulates seed germination and seedling growth. SMAX1 is a member of an uncharacterized family of eight genes in the model plant Arabidopsis thaliana. In this project genetic and biochemical approaches will be used to determine 1) how other genes in the SMAX1 family contribute to plant development; 2) the molecular function and regulation of SMAX1 protein; and 3) the protein interacting partners of SMAX1 and MAX2. These experiments will reveal how the SMAX1 family acts in karrikin and strigolactone signaling and whether SMAX1 is a target of MAX2. A postdoctoral researcher,graduate student, and several undergraduates will be trained for careers in science-related fields. Two projects will be initiated to improve undergraduate retention in science-related careers and enhance K-12 STEM education. The discoveries from this project will be disseminated through publications in peer-reviewed journals and presentations at scientific conferences.

David C Nelsonpropos ios-1350561Career：拟南芥中的Karrikin和Strigolactone信号传导机制是最近在烟雾中发现的一类化合物，它们在火灾后激活许多植物物种的发芽。斯特龙酮包括一个调节芽分支，根建筑和坎比亚生长的植物激素家族。斯特龙酮还引发了高度破坏性的寄生杂草，扫帚和巫婆的发芽，并促进了与羊膜菌根真菌的共生相关性。了解Karrikins和Strigolactones控制植物生长的分子机制可能会在作物发育和杂草控制中产生新的创新浪潮。植物通过需要F-box蛋白Max2的遗传途径识别Karrikins和Strigolactones。 F-box蛋白在几种植物激素信号系统中具有核心作用，并通过靶向特定的蛋白质底物降解来起作用。长期以来，Max2的目标一直难以捉摸，因此代表了了解Karrikin和Strigolactone信号传导机制的关键障碍。现在，遗传筛选已导致鉴定候选靶标，即Max2 1（Smax1）的抑制剂，该抑制剂调节种子发芽和幼苗生长。 Smax1是模型植物拟南芥中八个基因的未表征家族的成员。在这个项目中，将使用遗传和生化方法来确定1）SMAX1家族中的其他基因如何促进植物发育； 2）SMAX1蛋白的分子功能和调节； 3）SMAX1和MAX2的蛋白质相互作用伴侣。这些实验将揭示SMAX1家族在Karrikin和Strigolactone信号传导中的作用以及SMAX1是否是Max2的目标。博士后研究员，研究生和几名本科生将接受与科学相关领域的职业培训。将启动两个项目，以改善与科学相关职业的本科保留率并增强K-12 STEM教育。该项目的发现将通过科学会议的同行评审期刊和演讲中的出版物进行传播。