Mechanisms of SMXL6, 7 and 8 proteins in Arabidopsis plant adaptation to drought

SMXL6、7、8蛋白在拟南芥植物适应干旱的机制

基本信息

批准号：
20K05871
负责人：
TRAN LamSon・P
金额：
$ 2.75万
依托单位：
Institute of Physical and Chemical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2020
资助国家：
日本
起止时间：
2020-04-01 至 2021-03-31
项目状态：
已结题

项目摘要

We studied the functions of the strigolactone-signaling negative regulators SMXL6,7 and 8 in drought tolerance and the associated mechanisms by analyzing the drought-tolerant phenotype of the Arabidopsis thaliana triple smxl6,7,8 mutant plants and studied several drought tolerance-related traits. Our results together indicate that the SMXL6, 7 and 8 act as negative regulators of drought tolerance. The findings of this study suggest that disruption of these SMXL genes in crops may provide a novel way to improve their drought tolerance.Consistent with the results of the above study, strigolactone and karrikin receptors, DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2), respectively, have been shown to positively regulate drought tolerance in Arabidopsis. We then aimed to identify genes specifically or commonly regulated by D14 and KAI2 under drought, using comparative analysis of the transcriptome data of the A. thaliana d14-1 and kai2-2 mutants under dehydration conditions. Results suggest that some genes involved in cytokinin and brassinosteroid metabolism might be specifically regulated by the D14 pathway, whereas some genes related to photosynthesis and metabolism of glucosinolates and trehalose are potentially regulated by both D14 and KAI2 pathways in plant response to dehydration.

我们通过分析拟南芥thaliana thaliana thaliana triple smxl6，7,7,8突变植物的耐旱表型，研究了striglactone smxl6,7和8在干旱耐受性和相关机制中的功能SMXL6,7和8的功能。我们的结果共同表明，SMXL6、7和8充当干旱耐受性的负调节剂。这项研究的结果表明，农作物中这些SMXL基因的破坏可能提供了一种新的方法来提高其干旱耐受性。与上述研究的结果，Strigolactone和Karrikin受体的结果，dwarf14（D14）（D14）（D14）（D14）和Karrikin无敏感2（KAI2），分别显示了对Aright Poltight condististic plotightist antive drobistiss conteristiss plotistists protistist protistist antive drobistiss。然后，我们旨在使用在脱水条件下对D14和KAI2在干旱下特异或通常由D14和KAI2调节的基因，并使用A. thaliana D14-1和Kai2-2突变体的转录组数据进行比较分析。结果表明，某些与D14途径相关的细胞分裂素和黄铜代谢涉及的基因可能会特别调节，而某些与葡萄糖醇和心形成型光合作用和新代谢相关的基因可能受到D14和KAI2途径在植物响应脱氢中的响应的可能调节。