BRIGE: Metabolic Cell-Process Engineering (MCPE) for High Biobutanol Production by Clostridium tyrobutyricum

BRIGE：酪丁酸梭菌高生物丁醇生产的代谢细胞过程工程 (MCPE)

• 批准号: 1342390
• 负责人: Xiaoguang Liu
• 金额: $ 17.46万
• 依托单位: University of Alabama Tuscaloosa
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1342390&HistoricalAwards=false
• 关键词: BRIGE; Metabolic; Cell; Process; Engineering

The proposed research deals with engineering strategies to improve biobutanol production from low-cost, bacteria-based biomass technologies. Technical Description: This project seeks to achieve high-titer and high-yield butanol production by the bacterium Clostridium tyrobutyricum. Particularly, an innovative engineering approach, i.e., Metabolic Cell-Process Engineering (MCPE), will be developed to substantially increase biobutanol production through understanding the interaction between a host cell and its fermentation process. Specifically, it is planned to: 1) Develop a fundamental understanding of the response of a recently metabolically engineered C. tyrobutyricum mutant cell to various chemicals (e.g., electron carriers and inorganic salts), and identify cost effective chemicals to boost the NADH (Reduced Nicotinamide Adenine Dinucleotide) pool and detoxify the inhibitors from biomass hydrolysate. A Design of Experiment (DOE) approach will be used to screen and identify the chemicals needed for redox balance and detoxification. 2) Double butanol production via MCPE by increasing intracellular NADH and reducing the toxicity of biomass hydrolysate. The construction of a novel redox engineered mutant (MCE) and development of fermentation process with timed addition of the identified chemicals (MPE) will be integrated and utilized in butanol production.Broader Significance and Importance: Butanol is an important industrial solvent and a safe alternative transportation fuel that can be dispersed through existing pipelines and filling stations. Over the past decades, intensive efforts have been made to produce low-cost biobutanol using low-value biomass. However, biobutanol production still suffers from low yields and effective concentrations due to a variety of chemical issues associated with existing processes. The proposed MCPE engineering strategy has the potential to double biobutanol production, at a projected cost under $2.5 per gallon. Therefore, successfully developing the proposed technology can serve the public interest by providing a safe, renewable energy source while protecting natural resources and the environment. Many bioenergy industries and other areas of academic research would benefit from the development of novel engineering strain using MCPE.Broadening Participation of Underrepresented Groups in Engineering: This project also aims to broaden the participation of underrepresented groups and increase diversity. The Department of Chemical and Biological Engineering (ChBE) with over 600 undergraduates has historically attracted about 40 % women and more than 60 % of the students are interested in Biotech industry and graduate programs. Therefore, it is very important to enhance the ChBE curriculum by taking advantage of the unique 6-year Biotech industrial experiences of the PI. Specifically, a serial of bioproduction unit operations (e.g. novel bioreactor design and biobutanol fermentation demo run) will be designed and introduced to the traditional ChBE 319 Unit Operation Lab course. These new features can expand the students' knowledge of industry bioproduction and benefit their career goal development. Suitable biochemical reaction and titration experiments and guest lectures will be introduced to K-12 students through Alabama Science in Motion (ASIM) that covers 9 counties in west Alabama, including the impoverish Black Belt area. Outreach activities such as recruiting women researchers through SWE and REU and mentoring them in PI lab are also planned. This research has been funded through the Broadening Participation Research Initiation Grants in Engineering solicitation, which is part of the Broadening Participation in Engineering Program of the Engineering Education and Centers Division.

拟议的研究涉及工程策略，以改善低成本，基于细菌的生物质技术的生物丁醇生产。 技术描述：该项目旨在通过细菌蛋白酶蛋白酶来实现高滴和高收益的丁醇生产。特别是，通过了解宿主细胞与其发酵过程之间的相互作用，将开发一种创新的工程方法，即代谢细胞过程工程（MCPE），从而大大增加生物丁醇的产生。具体而言，它计划以下计划：1）对最近代谢性的C. prolobutyricum c。突变细胞对各种化学物质（例如电子载体和无机盐）的反应有基本的理解，并确定具有成本效益的化学物质，并确定促进NADH（减少烟碱氨基氨基核酸酯）的基本化学物质，并从Biox cool氧化物质化。实验方法（DOE）的设计将用于筛选和识别氧化还原平衡和排毒所需的化学物质。 2）通过增加细胞内NADH并降低生物质水解产物的毒性，通过MCPE产生双丁醇。将新型氧化还原工程突变体（MCE）的构建和发酵过程的开发与定时添加确定的化学药品（MPE）的定时构建将被整合和利用在丁醇生产中。Boader的重要性和重要性：丁醇是重要的工业溶剂，并且可以通过现有的管道和填充的站进行分散的安全替代运输燃料。在过去的几十年中，已经做出了强烈的努力，以使用低价值生物质生产低成本的生物丁醇。但是，由于与现有过程相关的多种化学问题，生物丁醇的产量仍然遭受较低的产量和有效浓度。拟议的MCPE工程策略有可能以每加仑2.5美元的价格生产生物丁醇生产两倍。因此，成功地开发拟议的技术可以通过提供安全，可再生能源的源头，同时保护自然资源和环境，从而为公共利益提供服务。许多生物能源行业和其他学术研究领域将受益于使用MCPE的新工程压力的发展。促进代表性不足的团体在工程中的参与：该项目还旨在扩大代表性不足的群体的参与并增加多样性。具有600多名本科生的化学与生物工程系（CHBE）历史上吸引了约40％的女性，而60％以上的学生对生物技术行业和研究生课程感兴趣。因此，通过利用PI的独特的6年生物技术工业经验来增强CHBE课程非常重要。具体而言，将设计并介绍传统的CHBE 319单元操作实验室课程并介绍生物生产单元操作的系列（例如新型生物反应器设计和生物丁醇发酵演示运行）。这些新功能可以扩大学生对行业生物生产的了解，并使他们的职业目标发展受益。将通过阿拉巴马州科学（ASIM）向K-12学生介绍合适的生化反应和滴定实验和客座讲座，该运动涵盖了西阿拉巴马州9个县，包括贫穷的黑带地区。还计划了外展活动，例如通过SWE和REU招募女性研究人员并在PI实验室指导她们。 这项研究是通过扩大参与研究的启动授予工程招标的资金的，这是扩大参与工程教育和中心部门工程计划的一部分。