MRI:Acquisition of an Isothermal Titration Calorimeter to Characterize Molecular Interactions

MRI：获取等温滴定量热计来表征分子相互作用

• 批准号: 1337721
• 负责人: David Rovnyak
• 金额: $ 9.1万
• 依托单位: Bucknell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337721&HistoricalAwards=false
• 关键词: MRI; Acquisition; Isothermal; Titration; Calorimeter

With this award from the Chemistry Major Research Instrumentation Program, Professor David Rovnyak from Bucknell University and colleagues Thomas Selby, Marie Pizzorno, Timothy Strein and Charles Clapp will acquire an isothermal titration calorimeter (ITC). The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) study of molecular interactions by microscale calorimetry; (b) guest-host binding by bile micelles; (c) study of protein-protein interactions between viral proteins; (d) characterization of inhibitors of soybean lipoxygenase-1; and (e) binding and specificity of phosphoinositide-specific phospholipase C (PI-PLC) enzymes.Isothermal titration calorimetry (ITC) is a very sensitive thermodynamic technique that is used to characterize binding interactions between species such as those between a small molecule and an enzyme. ITC measures directly the heat absorbed or released during a binding process. This provides insight on the strength of chemical and biochemical binding interactions. The calorimeter will also be incorporated into several upper division level laboratory classes. Thus this instrument will be used not only in research but also in educational and outreach activities.

在化学主要研究工具计划的奖项中，巴克内尔大学的David Rovnyak教授和同事Thomas Selby，Marie Pizzorno，Timothy Strein和Charles Clapp将获得等温滴定热量计（ITC）。该提案旨在增强各个层次的研究和教育，尤其是在（a）微观量热法对分子相互作用的研究中； （b）胆汁胶束的来宾主持人绑定； （c）研究病毒蛋白之间的蛋白质蛋白质相互作用； （d）表征大豆脂氧酶-1的抑制剂； （e）磷酸肌醇特异性磷脂酶C（PI-PLC）酶的结合和特异性。等热滴定量热法（ITC）是一种非常敏感的热力学技术，用于表征物种之间的结合相互作用，例如小分子和小分子和一个小分子之间的结合相互作用酶。 ITC在结合过程中直接测量吸收或释放的热量。这提供了对化学和生化结合相互作用的强度的见解。量热计还将被纳入几个上层级实验室类别。因此，该工具不仅将用于研究，还将用于教育和外展活动。