MRI: Acquisition of an Isothermal Titration Calorimeter for the Characterization of Molecular Interactions to Support Research and Undergraduate Education

MRI：购买等温滴定量热仪用于表征分子相互作用以支持研究和本科教育

• 批准号: 1531247
• 负责人: Veronica Moorman
• 金额: $ 9.16万
• 依托单位: Kettering University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531247&HistoricalAwards=false
• 关键词: MRI; Acquisition; Isothermal; Titration; Calorimeter

With this award from the Major Research Instrumentation (MRI) Program (MRI) and the Chemistry Research Instrumentation and Facilities (CRIF) Program, Kettering University will acquire an isothermal scanning calorimeter (ITC). Isothermal titration calorimetry has become a powerful technique for obtaining quantitative thermodynamic information about molecular interactions. It does so by sensitively measuring heat generated or absorbed by a compound upon titration using another compound. This leads to understanding the strength and structure of the interaction between the compounds. Binding between proteins, nucleic acids, lipids, small molecules, metals, nanoparticles, and polymers dissolved or suspended in aqueous or select non-aqueous solvents are accessible, making ITC extremely versatile. At Kettering University, the instrument will also be used by undergraduate students in their research projects. It will be incorporated into laboratory courses to provide training to students in several disciplines. It will be used in demonstrations and in hands on usage in outreach programs for middle and high school students. The proposal is aimed at enhancing research especially in areas such as (a) investigating protein-small molecule binding to explain the molecular evolution of the Yersina phosphatase YopH; (b) elucidating potential cytochrome c-dependent cardiovascular disease protection mechanisms for catechins, protein-metal complex binding; (c) determining the affinity of blood serum proteins for anti-cancer Ru(II)-mono-arene complexes; (d) determining protein-RNA binding for RNA sequence specificity of a T. brucei protein known to regulate gene expression and (e) studying small molecule-nanoparticle binding to understand the thermal properties of sodium acetate trihydrates for their use in non-electric incubating blankets.

通过主要研究工具（MRI）计划（MRI）和化学研究工具和设施（CRIF）计划的奖项，Kettering University将获得等温扫描量热仪（ITC）。等温滴定量热法已成为一种强大的技术，用于获得有关分子相互作用的定量热力学信息。它通过使用另一种化合物在滴定后敏感地测量化合物产生或吸收的热量来做到这一点。这导致理解化合物之间相互作用的强度和结构。蛋白质，核酸，脂质，小分子，金属，纳米颗粒以及溶解或悬浮在水性或精选的非水溶剂中的聚合物之间的结合，使ITC非常通用。在Kettering大学，本科生还将在其研究项目中使用该乐器。它将被纳入实验室课程中，以向几个学科的学生提供培训。它将用于中学和高中生的外展计划中的示范和使用中。该建议旨在增强研究，尤其是在（a）研究蛋白质 - 小分子结合以解释Yersina磷酸酶Yoph的分子演化的领域； （b）阐明潜在的细胞色素C依赖性心血管疾病保护机制，蛋白质 - 金属复合物结合； （c）确定血清蛋白对抗癌RU（II） - 单一雌激素络合物的亲和力； （d）确定蛋白-RNA的结合，以使已知的基因表达的T. brucei蛋白的RNA序列特异性和（e）研究小分子 - 纳米颗粒结合以了解乙酸钠三氢化物的热特性，以便它们在非电孵化毯中使用。