MRI: Acquisition of a small volume isothermal titration calorimeter

MRI：获取小体积等温滴定量热计

• 批准号: 1337676
• 负责人: Martin St. Maurice
• 金额: $ 10.25万
• 依托单位: Marquette University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337676&HistoricalAwards=false
• 关键词: MRI; Acquisition; small; volume; isothermal

An award is made to Marquette University for the acquisition of a small volume isothermal titration calorimeter (ITC) for the purpose of supporting research and education in the departments of Biological Sciences and Chemistry. ITC allows simultaneous measurements of multiple binding parameters in a single experiment, allowing quantitative thermodynamic analyses of biomolecular interactions in solution. A highly sensitive, small-volume instrument is particularly amenable to research in the life sciences, where sample preparation is time-consuming and expensive. This award will enhance research efforts aimed at (a) characterizing binding interactions between proteins and small molecules that will reveal new mechanistic details for metabolic and cell signaling enzymes; (b) characterizing protein:protein, protein:nucleotide and protein:lipid interactions in regulatory systems involved in maintaining cellular homeostasis; (c) measuring detailed thermodynamic profiles of biomolecular binding events in order to complement and enhance existing spectroscopic methods. This award will provide new instrumentation to support biochemical and biophysical research programs at both Marquette University and Concordia University, and will expand the biophysical infrastructure available to the broader Milwaukee research community. The ITC instrument will support educational initiatives at Marquette University through its incorporation into existing advanced laboratory courses in biochemistry and biophysics. It will also be accessible to students participating in undergraduate research programs in both biology and chemistry. The ITC instrument is easy to operate, allowing undergraduate students to gain first-hand experience in obtaining and interpreting thermodynamic data that are central to modern biochemistry and biophysics.

为了支持生物科学和化学部门的研究和教育目的，授予马奎特大学的奖励，以获取少量等温滴定量热量表（ITC）。 ITC允许在单个实验中同时测量多个结合参数，从而可以对溶液中生物分子相互作用进行定量热力学分析。一种高度敏感的小体积仪器特别适合在生命科学中的研究，在生命科学中，样本制备耗时且昂贵。该奖项将加强旨在（a）表征蛋白质与小分子之间的结合相互作用的研究工作，这些相互作用将揭示代谢和细胞信号酶的新机械细节； （b）表征蛋白质：蛋白质，蛋白质：核苷酸和蛋白质：与维持细胞稳态有关的调节系统中的脂质相互作用； （c）测量生物分子结合事件的详细热力学特征，以补充和增强现有的光谱方法。该奖项将提供新的工具，以支持Marquette University和Concordia University的生物化学和生物物理研究计划，并将扩大密尔沃基更广泛的密尔沃基研究社区可用的生物物理基础设施。 ITC工具将通过将其在生物化学和生物物理学的现有高级实验室课程中纳入Marquette University的教育计划。参加生物学和化学的本科研究计划的学生也将获得它。 ITC仪器易于操作，使本科生可以获得在获得和解释现代生物化学和生物物理学核心的热力学数据方面的第一手经验。