Modular Transcriptional Coordination of Central Metabolism

中枢代谢的模块化转录协调

• 批准号: 1330337
• 负责人: Daniel Kliebenstein
• 金额: $ 107.48万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1330337&HistoricalAwards=false
• 关键词: Modular; Transcriptional; Coordination; Central; Metabolism

Primary metabolism functions to provide the energy and building blocks for life and reproduction. Even with this central role, very little is known about how primary metabolism is regulated, especially at the level of gene expression. This project will employ a systematic approach to develop models of how this regulation occurs. A series of experimental strategies is expected to identify a set of key regulatory modules that are predicted to be central for the control of primary metabolism. Mutants of select transcription factors will be used to assess the phenotypic consequences using a high-throughput platform. Physiological, metabolomic and transcriptional effects will be monitored to assess module function. The combined data generated will enable further experimental studies to test whether regulatory modules of primary metabolism are structured around specific biochemical networks, or whether they are structured around physiological outputs such as increased growth or yield.Intellectual Merit: Modern biology has a fundamental desire to understand how the genome of an organism programs its phenotype. However, this is greatly complicated by the need to move from the molecular to the macroscopic scale. It requires critical information of how transcriptional regulation and primary metabolism are interconnected and how regulatory modules connect at the molecular and physiological scales. Current biochemical and systems dogma says that gene regulation is structured around biosynthetic pathways; however this project will directly test an alternative hypothesis that modules have a more interconnected structure built around the physiological endpoints. Finally, this project will begin to develop a predictive model of how the genome governs the dynamics of primary metabolism.Broader Impacts: A better understanding of how primary metabolism is regulated enables downstream manipulation of the process to engineer improvements in crop yield or quality. The project will provide interdisciplinary research opportunities for high school, undergraduate, and graduate students to prepare them for future careers in industry or academia. Established outreach programs will be used to recruit minority students from local high schools and colleges throughout the USA for summer internships. In addition, the principal investigators will be involved in teaching, both in a university classroom setting and in ongoing outreach efforts to educate community members about plant metabolism, quantitative genetics and ecology.

原发性代谢功能可为生命和繁殖提供能量和基础。即使有这种核心作用，对原代新陈代谢的调节是什么，尤其是在基因表达水平上，知之甚少。该项目将采用系统的方法来开发该法规的发生模型。预计一系列实验策略将确定一组关键的调节模块，这些模块被预测是控制原代新陈代谢的核心。精选转录因子的突变体将使用高通量平台评估表型后果。将监测生理，代谢组和转录效应以评估模块功能。生成的合并数据将使进一步的实验研究能够测试原代新陈代谢的调节模块是否围绕特定的生化网络构成，或者它们是否围绕着生长的生长或产量等生理产量进行结构。InteltectualForecit。生物体的基因组如何编程其表型。但是，这是从分子转移到宏观尺度的需要，这非常复杂。它需要有关转录调控和原代新陈代谢如何相互联系以及调节模块如何在分子和生理尺度上连接的关键信息。当前的生化和系统教条说，基因调节是围绕生物合成途径构成的。但是，该项目将直接检验一个替代假设，即模块具有围绕生理终点的更相互联系的结构。 最后，该项目将开始开发一个预测模型，说明基因组如何控制原发代谢的动态。Boader的影响：更好地理解原始代谢如何受到调节，使该过程的下游操纵能够在作物产量或质量方面改善工程师。该项目将为高中，本科生和研究生提供跨学科的研究机会，以为行业或学术界的未来职业做好准备。 既定的外展计划将用于招募来自美国当地高中和大学的少数民族学生进行暑期实习。此外，首席研究人员将参与教学，无论是在大学课堂环境中，以及正在进行的外展工作，以教育社区成员有关植物代谢，定量遗传学和生态学的教育。