Confocal microscopy at Hopkins Marine Station

霍普金斯海洋站的共焦显微镜

• 批准号: 1227226
• 负责人: Christopher Lowe
• 金额: $ 34.44万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1227226&HistoricalAwards=false
• 关键词: Confocal; microscopy; Hopkins; Marine; Station

Stanford University has been awarded a grant to purchase a confocal scanning laser microscope and establish a related multi-user facility at Hopkins Marine Station (HMS) to serve multiple marine laboratories in the Monterey Bay Peninsula, California. High-resolution imaging is now an essential part of the contemporary tool kit of cell and developmental biological processes. Confocal microscopy, along with advances in fluorescent labeling of protein and nucleic acids, has resulted in unprecedented insights into the 3D architecture and function of cells and tissues of many marine organisms. An important application of this technology is visualization of cellular elements and processes related to early development, physiology, and neurobiology. This technical ability also has broader implications for elucidating the interactions of marine animals with their environment. Ultimately, the environment acts on organisms through its effects on cells and tissues. Ocean acidification, deoxygenation and other processes will alter ecosystems and fisheries through effects on individual organisms. This microscope will help address how the various facets of climate change are impacting critical aspects of the cell/developmental biology and physiology of a variety of marine species. This piece of equipment will directly benefit 5 research labs at HMS with an immediate potential benefit to 10 graduate students, and provide outstanding undergraduate training opportunities during research training apprenticeships. The microscope will be housed at HMS but be available to researchers and their students at other research institutions in the local area, including the Monterey Bay Aquarium, Monterey Bay Aquarium Research Institute at Moss Landing and California State University Monterey Bay (CSUMB). There are many faculty at these institutions with a wide range of research interests who plan to use this microscope upon availability. HMS will partner with CSUMB Bay to create training opportunities on this microscope for the McNair Scholars program for gifted minority students to prepare them for doctoral studies. The image data generated from this equipment will be highlighted in the regular public lectures and open houses at HMS, and on a web-based resource dedicated to early embryogenesis and larval development of marine invertebrates. An imaging collaboration between HMS and the Monterey Bay Aquarium (MBA) will develop a greater public awareness of the diversity of embryos and larvae of marine invertebrates by spotlighting image data using the extensive established outreach mechanisms of the MBA both in formal public exhibits and Facebook postings. For more information about Hopkins Marine Station, visit the website at http://www-marine.stanford.edu/.

斯坦福大学（Stanford University）获得了购买共聚焦扫描激光显微镜的赠款，并在霍普金斯海军陆战队（HMS）建立相关的多用户设施，以服务于加利福尼亚州蒙特雷湾半岛的多个海洋实验室。现在，高分辨率成像是当代细胞和发育生物过程工具套件的重要组成部分。共聚焦显微镜以及蛋白质和核酸的荧光标记的进步，导致了对许多海洋生物体细胞和组织的3D结构和功能的前所未有的见解。 该技术的一个重要应用是与早期发展，生理学和神经生物学有关的细胞元素和过程的可视化。这种技术能力也对阐明海洋动物与环境的相互作用具有更广泛的意义。最终，环境通过其对细胞和组织的影响作用于生物体。 海洋酸化，脱氧和其他过程将通过对各个生物的影响改变生态系统和渔业。该显微镜将有助于解决气候变化的各个方面如何影响细胞/发育生物学的关键方面以及多种海洋物种的生理学。这套设备将直接受益于HMS的5个研究实验室，对10名研究生具有直接的潜在利益，并在研究培训学徒期间提供出色的本科培训机会。显微镜将安置在HMS，但可供当地其他研究机构的研究人员及其学生使用，包括蒙特雷湾水族馆，Moss Landing和加利福尼亚州立大学蒙特利湾（CSUMB）的蒙特雷湾水族馆研究所。在这些机构中，有许多具有广泛研究兴趣的教师计划在可用性时使用此显微镜。 HMS将与Csumb Bay合作，为麦克奈尔学者计划为有天赋的少数族裔学生创造培训机会，以便为博士生做准备。该设备产生的图像数据将在HMS的常规公开讲座和开放式房屋中突出显示，以及用于海洋无脊椎动物的早期胚胎发生和幼虫发展的基于网络的资源。 HMS与蒙特雷湾水族馆（MBA）之间的成像合作将提高公众对海洋无脊椎动物胚胎和幼虫多样性的认识，通过使用MBA在正式的公开展览和Facebook帖子中的广泛固定的外展机制来关注图像数据。有关Hopkins Marine Station的更多信息，请访问该网站http://www-marine.stanford.edu/。