DISSERTATION RESEARCH: Phylogeographic discord in the comparative genomic history of Amazonian birds

论文研究：亚马逊鸟类比较基因组历史中的系统地理学不一致

• 批准号: 1210556
• 负责人: Robb Brumfield
• 金额: $ 1.5万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-15 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1210556&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Phylogeographic; discord; comparative; DISSERTATION; RESEARCH; Phylogeographic; discord; comparative

The production of species diversity is of fundamental interest, but the factors that cause diversification are poorly understood. Diversification is known to occur at different rates depending on the species or group of species involved, and comparative study of this variation can reveal the processes that promote diversity. Comparative phylogeography is the study of diversification at the population level within different species, and it allows us to observe differences in diversification at the point where speciation occurs. The goals of this study are to use comparative phylogeography and novel methods of generating data to detect differences in diversification across species, and to analyze the factors that promote faster diversification in some species than in others. Phylogeography will be compared across sixteen Amazonian bird species with similar distributions. A new set of genetic markers scattered across the genome will be used to generate data from 24 individuals of each species. These genetic sequences will be extracted from museum tissue samples using a new method of enriching genomic DNA for sequences of interest, and then sequenced on a next-generation sequencing instrument. New analytical tools for testing models will allow us to measure the processes that are most important in driving the diversification of a given species. The massive amount of data generated by this method will allow for analysis of more complex models than could ever be analyzed previously, giving us new insight into the processes promoting species diversity. This project provides research opportunities for undergraduate students in Louisiana and the geographic scope of the project fosters inter-institutional and international collaborations. The research provides a test of next-generation sequencing methods and a new genomic marker set, and our results will aid in the application of these methods to other study systems. New biogeographic and geographic genetic data from our work support conservation efforts in the Amazon. All specimens, audio recordings, and observational data from our fieldwork and genetic data from our lab work are deposited in publicly accessible collection and databases. We consider these resources invaluable for current and future, pure and applied research on Amazonian birds and other systems.

物种多样性的产生具有根本的兴趣，但是引起多样化的因素对了解不足。已知多元化以不同的速度发生，具体取决于所涉及的物种或群体，并且对这种变化的比较研究可以揭示促进多样性的过程。比较植物地理学是对不同物种内人群水平的多样化的研究，它使我们能够观察到物种物种发生的程度差异。这项研究的目标是使用比较植物地理学和生成数据的新方法来检测各种物种多元化的差异，并分析促进某些物种比其他物种更快多样化的因素。将在十六种具有相似分布的亚马逊鸟类中比较植物地理学。散布在基因组上的一组新的遗传标记将用于从每个物种的24个个体中生成数据。这些遗传序列将使用一种富集基因组DNA的新方法从博物馆组织样品中提取，然后在下一代测序仪器上进行测序。用于测试模型的新分析工具将使我们能够衡量在推动给定物种多元化的过程中最重要的过程。该方法产生的大量数据将允许分析比以前可以分析的更复杂的模型，从而使我们对促进物种多样性的过程有了新的见解。该项目为路易斯安那州的本科生提供了研究机会，该项目的地理范围促进了机构间和国际合作。该研究提供了下一代测序方法和新的基因组标记集的测试，我们的结果将有助于将这些方法应用于其他研究系统。来自我们工作的新生物地理和地理遗传数据支持亚马逊的保护工作。从我们的实地调查和实验室工作中的遗传数据中的所有标本，录音和观察数据都存放在公共访问的收集和数据库中。我们认为这些资源对于当前和未来，对亚马逊鸟类和其他系统的纯粹和应用研究都有价值。