Doctoral Dissertation Research: Phylogeographic Investigation of Aedes aegypti

博士论文研究：埃及伊蚊的系统发育地理学调查

• 批准号: 2210879
• 负责人: Crystal Hepp
• 金额: $ 3.04万
• 依托单位: Northern Arizona University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2023-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2210879&HistoricalAwards=false
• 关键词: Doctoral; Dissertation; Research; Phylogeographic; Investigation; Doctoral; Dissertation; Research; Phylogeographic; Investigation

This project investigates the geographic range and movement of the mosquito vector Aedes aegypti in arid desert environments. Arid environments are of increasing attention for mosquito control as Ae. aegypti has recently and rapidly expanded into these arid environments. Ae. aegypti mosquitoes transmit dengue, yellow fever, Zika, and chikungunya viruses. This project sequences and analyzes the entire mitochondrial genome using phylogenetic techniques and couples these data with a spatially explicit analysis of phylogenetic patterns over two seasons. This analysis contributes to an understanding of which local conditions result in source populations and high individual counts. A more complete understanding of the underlying geographic distribution and movement of Ae. aegypti in arid desert environments is beneficial to vector control and public health efforts as this mosquito continues to expand its range and outbreaks of disease occur. This understanding supports public health interventions to be targeted efficiently and effectively to those at greatest risk by establishing a “range of risk” for Ae. aegypti-borne diseases.The objective of this project is to utilize whole mitochondrial genome sequencing of mosquito pools collected over two mosquito seasons to better understand the movement of the vector within this area, as well as any potential source locations of the mosquito population. Researchers will work with public health officials to procure geo-located mosquito samples from 2019 and 2020. Data will be sequenced and analyzed to produce the entire mitochondrial genome using various phylogenetic and phylogeographic techniques. This project develops an understanding of the geographic distribution and movement of Ae. aegypti and identifies potential source locations for the mosquito population in arid environments.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目调查了在干旱的沙漠环境中蚊子矢量埃及埃及的地理范围和运动。干旱环境对蚊子控制的关注越来越大。埃及最近已迅速扩展到这些干旱环境中。 Ae。埃及蚊子会传播风扇，黄热病，寨卡病毒和奇肯尼亚病毒。该项目序列序列并使用系统发育技术和伴侣对整个线粒体基因组进行了分析，并在两个季节内对系统发育模式进行了空间明确的分析。该分析有助于理解哪些当地条件导致来源人群和高度计数。对AE的基本地理分布和运动有了更完整的了解。在干旱的沙漠环境中的埃及对媒介的控制和公共卫生的努力有益，因为这种蚊子继续扩大其范围和疾病爆发，这种理解支持公共卫生干预措施，通过为AE建立“风险范围”，以有效地有效地对处于最大风险的人进行有效和有效的风险。该项目的目的是利用在两个蚊子季节收集的蚊子池的整个线粒体基因组测序，以更好地了解该区域内载体的运动，以及蚊子种群的任何潜在源地点。研究人员将与公共卫生官员合作，从2019年和2020年开始购买地理位置的蚊子样本。将使用各种系统发育和植物地理技术对数据进行测序并分析以生成整个线粒体基因组。该项目对AE的地理分布和运动有了了解。 Aegypti并确定了干旱环境中蚊子人口的潜在来源位置。该奖项反映了NSF的法定任务，并使用基金会的知识分子优点和更广泛的影响审查标准，认为通过评估值得支持。