An Automated Capillary Sequencer for Systematics, Population Genetics, Genomics, and Molecular Ecology

用于系统学、群体遗传学、基因组学和分子生态学的自动毛细管测序仪

• 批准号: 0400797
• 负责人: Robb Brumfield
• 金额: $ 12.65万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-15 至 2006-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0400797&HistoricalAwards=false
• 关键词: Automated; Capillary; Sequencer; Systematics; Population

This award provides support for acquisition of a modern capillary DNA sequencing instrument capable of determining nearly 200,000 bases a day at 98.5% accuracy, and also capable of high throughput fragment analysis including that of microsatellites and single nucleotide polymorphisms. The instrument will be used in a number of ongoing projects, including an NSF-funded Tree of Life sequencing project aimed at producing a molecular phylogeny of all birds. The instrument will be used. In addition to its role in research, the instrument will be used for training at the high school, undergraduate, graduate, and post-doctoral levels. The instrument will replace an existing slab-gel instrument that has been heavily used for studies of population genetics and evolutionary genomics of avian systems, co-evolutionary genetics of mammals and their parasitic lice, and evolutionary genetics of reptiles and amphibians. The new instrument will provide a several-fold increase in the sequencing capacity that can be brought to bear on these and other projects at the Museum of Natural History.

该奖项为获得现代毛细管DNA测序仪器的获取提供了支持，能够以98.5％的精度确定近200,000个基础，并且还能够进行高吞吐量片段分析，包括微卫星和单核苷酸多态性。 该仪器将用于许多正在进行的项目，包括由NSF资助的生命测序项目，旨在产生所有鸟类的分子系统发育。 该仪器将被使用。 除了其在研究中的作用外，该仪器还将在高中，本科，研究生和博士后水平进行培训。 该仪器将替代现有的平板 - 凝胶仪器，该仪器已大量用于研究禽类系统的人群遗传学和进化基因组学，哺乳动物及其寄生虫的共进化遗传学以及爬行动物和两栖动物的进化遗传学。 该新仪器将在自然历史博物馆的这些项目和其他项目中提供几倍的测序能力。