DISSERTATION RESEARCH: Phylogeographic analysis of introgressive gene flow among nuclear loci functionally linked to the mitochondrion

论文研究：与线粒体功能相关的核位点间渐渗基因流的系统地理学分析

• 批准号: 1406876
• 负责人: David Weisrock
• 金额: $ 1.95万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1406876&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Phylogeographic; analysis; introgressive

Genetic studies across species frequently uncover conflicting patterns between the nuclear and organellar (including mitochondria) genomes. Contrasting patterns of genomic divergence can potentially be explained by differences related to inheritance, gene flow, and mutation rate, but it is unknown how co-evolutionary interactions between these genomes influence genetic divergence. Using Dusky Salamanders and revolutionary DNA sequencing technologies, evolutionary patterns of divergence will be compared between the nuclear and mitochondrial genomes, with a focus on nuclear genes that function within the mitochondrion. Dusky salamanders are an excellent system for this project because they are regionally diverse in eastern North America and have demonstrated gene flow across species boundaries. These criteria, and the growing genetic resources available for salamanders, make them an excellent system to test for conflicting evolutionary patterns between nuclear and nuclear-encoded mitochondrial genes.This research will contribute toward graduate student training in the use of rapidly changing and advancing genetic technologies and help preparations for an academic career through peer-reviewed research and undergraduate mentorship. Results from this study will provide new insights into how researchers should use genomic data to reconstruct the Tree of Life, and will contribute to an understanding of the processes that influence genetic divergence and speciation.

跨物种的遗传研究经常发现核和细胞器（包括线粒体）基因组之间的冲突模式。基因组差异的对比模式可以通过与遗传，基因流和突变率有关的差异来解释，但是这些基因组之间的共同进化相互作用如何影响遗传差异。使用昏暗的sal和革命性的DNA测序技术，将在核和线粒体基因组之间比较差异的进化模式，重点是在线粒体内发挥作用的核基因。 Dusky Salamanders是该项目的绝佳体系，因为它们在北美东部地区是区域多样的，并且在物种边界上表现出基因流。这些标准以及可用于sal的遗传资源不断增长的遗传资源，使它们成为测试核和核编码的线粒体基因之间相互矛盾的进化模式的绝佳系统。这项研究将有助于研究生培训在快速变化和促进遗传技术的培训，并通过友善的研究生来为学术生涯提供良好的研究生培训。这项研究的结果将为研究人员如何使用基因组数据重建生命之树提供新的见解，并将有助于理解影响遗传差异和物种形成的过程。