CAREER: The influence of soil attachment on the biologic activity of extracellular proteins

职业：土壤附着对细胞外蛋白质生物活性的影响

• 批准号: 1149242
• 负责人: Shannon Bartelt-Hunt
• 金额: $ 41.39万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1149242&HistoricalAwards=false
• 关键词: CAREER; influence; soil; attachment; biologic

1149242-Bartelt-HuntExtracellular proteins have recently been implicated in a range of environmental engineering problems including horizontal transmission of prion diseases and the release of insecticidal toxins from transgenic plants. In addition, the increasing use of environmental proteomics necessitates a more complete understanding of the behavior of extracellular proteins in the environment. Despite this, the interactions between proteins and environmentally-relevant surfaces such as soil minerals are still poorly understood as is the influence attachment to soil can have on biologic properties of extracellular proteins. The research objective is to utilize a novel analytical tool to evaluate the interaction of one type of extracellular protein with environmentally-relevant surfaces, as well as to evaluate the subsequent changes in biologic function of the soil-attached protein. The study utilizes the new analytical tool to simultaneously investigate the amount and conformation of protein adsorbed to surfaces. In addition, the PI will perform a series of experiments to evaluate how attachment of proteins to soil can influence their biologic properties. The information generated in this research will further understanding of the role of the environment in transmission of chronic wasting disease, an emerging animal disease that currently has no effective therapeutic treatments. Results from this study will identify how extracellular proteins interact with soils, and will inform a range of environmental engineering issues including prion disease transmission, transport of extracellular enzymes released from transgenic crops, and the behavior of proteins in soil mineral cycles. Further, the project will provide cooperative learning materials in engineering potentially leading to improved student retention in engineering, and ultimately, a more robust and diverse engineering workforce. The PI will share findings with extension educators in states impacted by prion diseases and with K-12 math and science teachers in the state of Nebraska through the University of Nebraska?s Science and Math Professional Development Summer Technology Institute. The PI will involve undergraduate students in research, and will leverage funding from an existing University of Nebraska program to provide significant undergraduate involvement in the research activities described here.

1149242-bartelt-huntextracthactarular蛋白最近与一系列环境工程问题有关，包括水平传播prion疾病和从转基因植物中释放杀虫毒素。此外，环境蛋白质组学的越来越多的使用需要对环境中细胞外蛋白的行为有更全面的了解。尽管如此，蛋白质与与环境相关的表面（例如土壤矿物质）之间的相互作用仍然很鲜为人知，因为对土壤的影响附着对细胞外蛋白质的生物学特性也是如此。研究的目标是利用一种新型的分析工具来评估一种类型的细胞外蛋白与环境相关的表面的相互作用，并评估土壤连续蛋白的生物学功能的随后变化。该研究利用新的分析工具同时研究吸附到表面的蛋白质的量和构象。此外，PI将执行一系列实验，以评估蛋白质对土壤的附着如何影响其生物学特性。这项研究中产生的信息将进一步了解环境在慢性浪费疾病传播中的作用，这是一种新兴的动物疾病，目前尚无有效的治疗治疗。这项研究的结果将确定细胞外蛋白如何与土壤相互作用，并将为一系列环境工程问题提供信息，包括流传疾病的传播，从转基因作物释放的细胞外酶的运输以及蛋白质在土壤矿物质周期中的行为。此外，该项目将在工程学中提供合作学习材料，从而有可能改善学生在工程学领域的保留，并最终成为更强大，更多样化的工程劳动力。 PI将通过内布拉斯加州大学的科学和数学专业发展夏季技术研究所与受prion疾病影响的各州以及在内布拉斯加州州的K-12数学和科学教师产生影响的州的扩展教育者分享发现。 PI将让本科生参与研究，并利用现有的内布拉斯加州大学计划的资金来提供大量的本科参与此处描述的研究活动。