Collaborative Research: Fate and bioavailability of steroidogenic compounds in aquatic sediment

合作研究：水生沉积物中类固醇化合物的归宿和生物利用度

• 批准号: 0966850
• 负责人: Shannon Bartelt-Hunt
• 金额: $ 22.01万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0966850&HistoricalAwards=false
• 关键词: Collaborative; Research; Fate; bioavailability; steroidogenic

Proposal Title: Collaborative Research: Fate and bioavailability of steroidogenic compounds in aquatic sedimentPrincipal Investigators: Bartelt-Hunt, Shannon; Kolok, AlanInstitutions: University of Nebraska-Lincoln; University of Nebraska-OmahaProposal Nos: CBET-0966850; CBET-0966858 Environmental exposures to elevated levels of steroids have been linked with negative reproductive outcomes in fish and potential human health impacts including defective bone formation and impacts on brain development. Previously-published studies indicate that exogenous steroids will sorb to aquatic sediments, however the bioavailability of sediment-associated steroid hormones to aquatic organisms is not clear. The PIs? preliminary data suggest that sediment-associated steroids remain bioavailable and, moreover, may represent the principle source of bioavailable steroidogenic compounds in aquatic systems. The proposed work is innovative as it represents the first study to examine how association with sediment alters the bioavailability of steroids to aquatic biota. The PIs will examine the physical and chemical factors responsible for estrogen and androgen fate and transformation in sediment as well as the routes by which macroorganisms are exposed to biologically-active steroid hormones. The results of the proposed research will provide a better understanding of the role of sediment in steroid transformation and biologic uptake and will support design of effective mitigation strategies in aquatic systems impacted by steroidogenic compounds. The objective of this application is to examine the role that sediments play in the environmental fate, transformation and bioavailability of steroidogenic compounds. The central hypothesis of this study is that sediment-associated steroids remain bioavailable. They plan to test their central hypothesis and accomplish the objective of this application by pursuing the following three specific aims: (1) determine the exposure routes by which steroids become bioavailable to the fathead minnow, a ubiquitous freshwater fish, (2) evaluate the physicochemical mechanisms governing steroid in sediment, and (3) validate their laboratory results with controlled field studies conducted in aquatic systems with known steroidogenic discharge. The educational and outreach plan for this project is focused on undergraduate and graduate student education as well as providing information to regional, national and international groups with an interest in endocrine-disrupting compounds. The proposed project represents a collaborative research effort between faculty at a research I University (University of Nebraska-Lincoln) and a predominantly undergraduate institution (University of Nebraska at Omaha). Educational modules outlining the methods and results of this research will be integrated into existing courses focused on contaminant fate and transport, environmental sampling methods, and toxicology taught by project investigators at the University of Nebraska-Lincoln and the University of Nebraska at Omaha. Research results and information on hormones in the environment will be disseminated to wastewater treatment plant operators, to the public through articles published in a University of Nebraska quarterly publication and an interactive website designed for a lay audience. International outreach will be achieved through a graduate student exchange program with the Corporación para Investigaciones Biológicas and the University of Antioquia in Medellin, Columbia.

提案标题：合作研究：水生沉积物调查人员中类固醇化合物的命运和生物利用度：香农Bartelt-Hunt； Kolok，Alaninstitutions：内布拉斯加林肯大学；内布拉斯加大学 - 奥马帕斯大学NOS：CBET-0966850; CBET-0966858环境暴露于类固醇水平升高的环境暴露与鱼类的负面生殖结果以及潜在的人类健康影响有关，包括有缺陷的骨形成和对脑发育的影响。先前发表的研究表明，外源性类固醇会刺激到水生沉积物中，但是与沉积物相关的类固醇恐怖与水生生物的生物利用度尚不清楚。 pis？初步数据表明，与沉积物相关的类固醇仍然可生物利用，此外，可能代表了水生系统中可生物可利用的类固醇生成化合物的主要来源。拟议的工作具有创新性，因为它代表了第一个研究与沉积物的关联如何改变类固醇对水生生物植物的生物利用度的研究。 PI将检查负责雌激素和雄激素命运的物理和化学因素，以及沉积物中的转化，以及宏观生物暴露于生物活性类固醇恐怖的途径。拟议研究的结果将更好地理解沉积物在类固醇转化和生物学摄取中的作用，并将支持在受类固醇生成化合物影响的水生系统中设计有效缓解策略的设计。该应用的目的是检查沉积物在环境命运，类固醇生成化合物的转化和生物利用度中的作用。这项研究的核心假设是，与沉积物相关的类固醇仍然可生物利用。他们计划通过追求以下三个特定目的来检验其中心假设并实现此应用的目标：（1）确定类固醇可以生物可利用的暴露途径，该途径可用于Fathead Minnow，一种无处不在的淡水鱼（一种无处不在的淡水鱼，（2）评估毒液中的类固醇机构的物理机制，（3）与已知的实验室进行了验证，并与他们的实验室进行了验证。该项目的教育和外展计划的重点是本科生和研究生教育，并向对内分泌干扰化合物感兴趣的区域，国家和国际团体提供信息。拟议的项目代表了I大学研究（内布拉斯加州林肯大学）与主要本科机构（内布拉斯加州大学奥马哈大学）之间的合作研究。概述这项研究的方法和结果的教育模块将纳入专注于污染物和运输，环境抽样方法以及内布拉斯加州林肯大学和内布拉斯加州内布拉斯加州大学内布拉斯加州大学的项目调查员教授的毒理学。有关环境中激素的研究结果和信息将通过内布拉斯加州大学季度出版物上发表的文章和专为外行受众设计的互动网站传播给废水处理厂运营商，向公众传播。国际宣传将通过与哥伦比亚梅德林的Antioquia大学的CorporaciónParaRespectionesBiológicas和Antioquia大学一起实现。