Collaborative Research: pH-Responsive capsules for Enhanced Delivery and Recovery of Cellulases for Biomass Hydrolysis

合作研究：用于增强生物质水解纤维素酶输送和回收的 pH 响应胶囊

• 批准号: 0966526
• 负责人: Sergiy Minko
• 金额: $ 20.1万
• 依托单位: Clarkson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-15 至 2014-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0966526&HistoricalAwards=false
• 关键词: Collaborative; Research; pH; Responsive; capsules

0966526MinkoEffective, efficient, and economical conversion technologies are needed to meet the potential of using biobased feedstocks to produce liquid fuels and other high-value chemicals. Enzymatic hydrolysis of plant cell wall structural carbohydrates into soluble and fermentable sugars has been technically achievable for decades. Despite significant advances in the past five years, the economical production and use of cellulase enzymes for biomass hydrolysis remain key hurdles. This is true whether one is seeking hydrocarbons or economical cellulosic ethanol. The key to conquering these obstacles according to the collaborating PIs Minko of Clarkson University in Potsdam, NY and Voronov and Pryor of North Dakota State University at Fargo rests in placing the enzymes in nanostructured capsules. These carefully designed and fabricated, hybrid organic-inorganic microcapsules are loaded with a cocktail of cellulase enzymes for the conversion of cellulose into fermentable glucose. These unique capsules protect the enzymes and preserve their activity, allow for a simple reuse/recovery process for the enzymes, and provide an opportunity to regulate enzymatic reactions using external signals, such as pH. This enzyme recovery and reuse, facilitated through the encapsulation process and magnetic separation, are expected to have significant impacts on processing costs to produce biomass-derived sugars. The PIs plan to create a website as a means of sharing data and plans between the research groups at the two universities, and to allow public access to follow aspects of the project. They intend to utilize REU opportunities and high school researchers as a part of their outreach efforts. This is a challenging project for all of the researchers. There are many synthetic chemistry components that all must come together in the fabrication of the encapsulated enzymes. The successful development of reversible encapsulation technology for applications of enzymes would have far-reaching implications in a number of industrial processes and would constitute a significant advance in the field.

0966526Minko 需要有效、高效且经济的转化技术来满足使用生物基原料生产液体燃料和其他高价值化学品的潜力。将植物细胞壁结构碳水化合物酶促水解成可溶性和可发酵的糖在技术上几十年来一直是可以实现的。尽管过去五年取得了重大进展，但用于生物质水解的纤维素酶的经济生产和使用仍然是主要障碍。无论是寻找碳氢化合物还是经济的纤维素乙醇，这都是事实。纽约州波茨坦克拉克森大学的合作 PI Minko 以及法戈北达科他州立大学的 Voronov 和 Pryor 表示，克服这些障碍的关键在于将酶放入纳米结构胶囊中。这些精心设计和制造的有机-无机混合微胶囊装载有纤维素酶混合物，用于将纤维素转化为可发酵的葡萄糖。这些独特的胶囊可以保护酶并保持其活性，允许对酶进行简单的再利用/回收过程，并提供使用外部信号（例如 pH）调节酶反应的机会。通过封装过程和磁力分离促进的酶回收和再利用预计将对生产生物质衍生糖的加工成本产生重大影响。 PI 计划创建一个网站，作为两所大学研究小组之间共享数据和计划的手段，并允许公众了解该项目的各个方面。他们打算利用 REU 的机会和高中研究人员作为其外展工作的一部分。对于所有研究人员来说，这都是一个具有挑战性的项目。在制造封装酶时必须将许多合成化学成分组合在一起。用于酶应用的可逆封装技术的成功开发将对许多工业过程产生深远的影响，并将构成该领域的重大进步。