Isothermal Calorimetry in Physical Chemistry and Biochemistry Laboratories

物理化学和生物化学实验室中的等温量热法

• 批准号: 0941517
• 负责人: Leah O'Brien
• 金额: $ 19.9万
• 依托单位: Southern Illinois University at Edwardsville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-10-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0941517&HistoricalAwards=false
• 关键词: Isothermal; Calorimetry; Physical; Chemistry; Biochemistry

Chemistry (12) Laboratory experiments are being developed for upper-level Physical Chemistry and Biochemistry Laboratory courses that utilize isothermal titration calorimetry (ITC). The exercises include: "Binding Constants of EDTA with Ca(II) and Mg(II) Ions"; "Michaelis-Menten Enzyme Kinetic Study of the Hydrolysis of Benzoylarginine Ether Ester (BAEE) by Trypsin"; "Binding of the N-Acetylglucosamine Trisaccharide ((NAG)3)Ligand to Lysozyme"; and "Investigation of the Interaction of Calmodulin with Ca2+/Calmodulin Kinase II". The binding affinity, reaction enthalpy, and stoichiometric ratio for the two reagents are being obtained directly from the ITC experiments. Additionally, by combining these reaction parameters, it is also possible to determine the reaction entropy and the change in Gibb's free energy for the reaction studied. Program assessment will include the impact of these innovative educational materials on student learning.Isothermal titration calorimetry is a relatively new technique that is used to determine thermodynamic parameters for small scale reactions. The interdisciplinary nature of isothermal calorimetry makes it a powerful tool to demonstrate the link between discovery and societal benefits such as drug development. Modern, ultrasensitive ITC has found many applications in Biochemistry and Pharmaceutical research: protein-protein, protein-drug, protein-DNA, protein-ligand and metal-ligand interactions are examples of intermolecular interactions that can be studied. Educational materials resulting from these efforts are being nationally disseminated at chemistry education conferences and via manuscripts submitted to professional journals.

化学（12）实验室实验正在开发用于利用等温滴定量热法（ITC）的高级物理化学和生物化学实验室课程。 练习包括：“与Ca（II）和Mg（II）离子的EDTA的绑定常数”； “ Michaelis-Menten酶动力学研究了胰岛素的苯甲胺醚酯（BAEE）水解的研究”； “ N-乙酰葡萄糖三糖（（NAG）3）配体与溶菌酶的结合”；和“调查钙调蛋白与Ca2+/钙调蛋白激酶II的相互作用”。 这两种试剂的结合亲和力，反应焓和化学计量比直接从ITC实验中获得。 另外，通过结合这些反应参数，还可以确定反应熵和所研究反应的吉布自由能的变化。 计划评估将包括这些创新的教育材料对学生学习的影响。等热滴定量热法是一种相对较新的技术，用于确定小规模反应的热力学参数。 等温量热法的跨学科性质使其成为证明发现与药物开发等社会福利之间联系的有力工具。 现代，超敏感的ITC在生物化学和药物研究中发现了许多应用：蛋白质 - 蛋白质，蛋白药，蛋白质-DNA，蛋白质-DNA，蛋白质 - 配体和金属 - 配体相互作用是可以研究的分子间相互作用的实例。 这些努力产生的教育材料正在全国范围内在化学教育会议上以及通过提交专业期刊的手稿传播。