MRI: Acquisition of Instuments to Facilitate Molecular-Level Studies in Earth and Ocean Sciences

MRI：购置仪器以促进地球和海洋科学的分子水平研究

• 批准号: 0723222
• 负责人: Lihini Aluwihare
• 金额: --
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0723222&HistoricalAwards=false
• 关键词: MRI; Acquisition; Instuments; Facilitate; Molecular

The proposal requests funds for a gas-chromatograph time-of-flight mass spectrometer with a preparatory fraction collector and a liquid chromatograph-linear ion trap mass spectrometer with multiple sample ionization modes and an analytical fraction collector. The instrumentation will be used by the PI and her students to characterize and purify biomolecules from complex environmental samples for chemical, biological and geological research efforts. The facility will be used to support existing and proposed research efforts at SIO, including identification of membrane lipid and protein modifications associated with temperature and pressure tolerance of deep-ocean bacteria, and genomics enabled studies of membrane lipid biosynthesis. The facility will also be used to develop new analytical methods, and train graduate students in instrumentation operation and use. Use of the facility by outside users is by invitation.Broader impactsThe PI has a strong record of enhancing the participation of women in science by hosting female high school students. The proposed instrumentation will complement and expand the capabilities of SIO researchers, as well as invited outside researchers. Seminars will be held for either the Marine Chemistry and Geochemistry or Marine Biology seminar series demonstrating the facility capabilities and results to the larger SIO populace

该提案要求资金提供带有预备分数收集器的飞行时间质谱仪和液态色谱线性离子陷阱质谱仪，具有多种样品电离模式和分析分数收集器。 PI和她的学生将使用该仪器来表征和净化复杂环境样品的生物分子，以进行化学，生物和地质研究工作。 该设施将用于支持SIO的现有研究和拟议的研究工作，包括鉴定与深度海菌的温度和压力耐受性相关的膜脂质和蛋白质修饰，以及基因组学使膜脂质生物合成的研究。 该设施还将用于开发新的分析方法，并在仪器操作和使用方面培训研究生。 外部用户对设施的使用是受邀请。BroaderImpacts The Pi通过接待女性高中生，在增强妇女参与科学方面的参与度有很强的记录。 拟议的仪器将补充和扩大SIO研究人员的能力以及受邀外部研究人员的能力。 将举行研讨会的海洋化学和地球化学或海洋生物学研讨会系列，展示了较大的Sio Populace的设施能力和结果