Collaborative Research: Role of Meso- and Bathypelagic prokaryotic Organisms in the Marine Water Column: Insights from Compound-Specific Radiocarbon Analysis

合作研究：海洋水体中中层和深海原核生物的作用：化合物特异性放射性碳分析的见解

• 批准号: 0242160
• 负责人: Lihini Aluwihare
• 金额: $ 16.08万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0242160&HistoricalAwards=false
• 关键词: Collaborative; Research; Role; Meso; Bathypelagic

ABSTRACTOCE-0242160OCE-0241363Even though the meso- and bathypelagic regions beneath the euphotic zone contain nearly all of the oceans volume, little is known about the biogeochemical processes that function in this region. For this reason, a PI from Scripps Institute of Oceanography will collaborate with a PI from Harvard University to determine which carbon sources fuel prokaryotic production in the meso- and bathypelagic ocean. Specifically, the PIs wish to address the following two questions: (1) Is freshly-produced organic matter the primary dissolved organic carbon (DOC) pool that is accessible to deep ocean heterotrophic prokaryotes or is "old" DOC also bioavailable?; and (2) Is chemoautotrophic fixation of inorganic carbon by planktonic archaea an important component of the deep ocean carbon cycle? To address these questions, the PIs plan to measure the radiocarbon (14C) concentration of the carbon source pools and the resulting prolaryotic (bacterial and archaeal) biomass at the surface, meso (500-600 m) and bathypelagic (900 m) depths. In addition, fluorescent in-situ hybridization (FISH) and genetic diversity analysis by denaturing gradient gel electrophorsis (DGGE) will provide a complementary picture of the biological community. Lastly, to obtain the large quantities of biomass-derived carbon needed for this study, the PIs plan to develop an in-situ, battery powered, submersible pump capable of pumping 20,000L of seawater per deployment during the first year of the study.

Abstractoce-0242160OCE-0241363EVE，虽然在舒适区下方的中间和沐浴 - 层状区域几乎包含所有海洋量，但对在该区域起作用的生物地球化学过程几乎没有任何了解。 因此，Scripps海洋学研究所的PI将与哈佛大学的PI合作，以确定哪种碳源为中和乙型层海洋燃料核生产。 具体而言，PI希望解决以下两个问题：（1）是新鲜生产的有机物，主要溶解的有机碳（DOC）池，可用于深海异嗜性原核生物，还是“旧的” doc也可以生物利用？ （2）通过浮游古细菌对无机碳的化学养分固定是深海碳循环的重要组成部分吗？ 为了解决这些问题，PIS计划测量碳源库的放射性碳（14C）浓度以及所得的佛罗里培训（细菌和古细菌）在表面，Meso，Meso（500-600 m）和沐浴型（900 m）深度。 此外，通过贬低梯度凝胶电泳（DGGE），荧光原位杂交（FISH）和遗传多样性分析将提供生物学群落的互补情况。 最后，为了获得这项研究所需的大量生物质衍生的碳，PIS计划开发一个原位，电池供电的潜水泵，能够在研究的第一年中每部部署20,000升海水。