CAREER: Low Coherence Light Scattering for Biophotonics

职业：生物光子学的低相干光散射

• 批准号: 0348204
• 负责人: Adam Wax
• 金额: $ 40万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0348204&HistoricalAwards=false
• 关键词: CAREER; Low; Coherence; Light; Scattering

0348204WaxThis CAREER Development Plan focuses on utilizing optics knowledge from the field of photonics for biomedical engineering applications. The goal of the research plan is to develop new applications of low coherence interferometry (LCI) for detecting light scattered by cells and tissues as a method of determining their structural and functional features with sub-wavelength sensitivity. Optical techniques offer the advantages of being non-invasive, non-ionizing and readily implemented by simple, cost-efficient means while avoiding the need for fixatives and staining agents and their associated artifacts. The research plan is based on three activities: 1. To further develop Angle-resolved LCI (a/LCI), a method for measuring cell morphometry in situ, the efficacy of which already has been validated in the laboratory. The a/LCI technique has great potential as an early-cancer diagnostic that will be realized by implementing and applying robust a/LCI instruments for both clinical and laboratory investigations of cells and tissues.2. The validation and development of Fourier-domain LCI, a novel method for probing sub-surface structure in biological tissues using optical spectroscopy. This approach is a simpler optical scheme than a/LCI but to date has only been demonstrated on tissue phantoms. Further experiments will help to evaluate its potential capabilities.3. The development of a novel microscopy scheme based on using an actively stabilized interferometer to measure the phase of scattered light for cell biology applications. This technique will enable precision measurements of the biophysical properties of cells. The education plan will incorporate photonics into the biomedical engineering curriculum by developing a new Biophotonics course and new teaching materials. The specific activities include development of a modern microscopy course, incorporation of Biophotonics into existing BME courses and development of independent Biophotonics projects for students ranging from high school to the graduate level. The activities will take advantage of a new Biophotonics teaching laboratory that will house state of the art equipment for instructional use. The education program also will benefit from the help of the outreach program at Duke University that will enable introduction of Biophotonics to high school students, through independent projects and to underrepresented groups by involving visiting students in research projects in the principal investigator's laboratory.

0348204Waxthis职业发展计划的重点是利用光子学领域的光学知识用于生物医学工程应用。 研究计划的目的是开发低相干干涉仪（LCI）的新应用，用于检测细胞和组织散射的光，以确定其具有亚波长灵敏度的结构和功能特征。光学技术提供了非侵入性，非电离和容易通过简单，具有成本效益的方式实施的优点，同时避免了对固定剂和染色代理的需求及其相关的工件。研究计划基于三个活动：1。为了进一步开发角度分辨的LCI（A/LCI），这是一种测量原位细胞形态计量学的方法，其功效已经在实验室中得到验证。 A/LCI技术作为早期癌症诊断的潜力很大，可以通过实施和应用强大的A/LCI仪器来实现细胞和组织的临床研究和实验室研究。2。 傅立叶域LCI的验证和开发，这是一种使用光谱法探测生物组织中地下结构的新方法。该方法比A/LCI更简单，但迄今为止仅在组织幻像上证明了这一方法。进一步的实验将有助于评估其潜在能力3。 基于使用积极稳定的干涉仪来测量细胞生物学应用散射光的相位的新型显微镜方案的开发。该技术将实现细胞生物物理特性的精确测量。该教育计划将通过开发新的生物探测课程和新的教材，将光子学纳入生物医学工程课程。具体的活动包括开发现代显微镜课程，将生物素化学纳入现有的BME课程以及为从高中到研究生层面的学生开发独立的生物探测项目。这些活动将利用一个新的生物素教学实验室，该实验室将容纳最先进的设备以供教学使用。该教育计划还将受益于杜克大学的外展计划的帮助，该计划将通过独立项目和代表性不足的群体向高中生引入生物素，并通过让拜访学生参与研究项目的研究项目。