CAREER: Low Coherence Light Scattering for Biophotonics

职业：生物光子学的低相干光散射

• 批准号: 0348204
• 负责人: Adam Wax
• 金额: $ 40万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0348204&HistoricalAwards=false
• 关键词: CAREER; Low; Coherence; Light; Scattering

0348204WaxThis CAREER Development Plan focuses on utilizing optics knowledge from the field of photonics for biomedical engineering applications. The goal of the research plan is to develop new applications of low coherence interferometry (LCI) for detecting light scattered by cells and tissues as a method of determining their structural and functional features with sub-wavelength sensitivity. Optical techniques offer the advantages of being non-invasive, non-ionizing and readily implemented by simple, cost-efficient means while avoiding the need for fixatives and staining agents and their associated artifacts. The research plan is based on three activities: 1. To further develop Angle-resolved LCI (a/LCI), a method for measuring cell morphometry in situ, the efficacy of which already has been validated in the laboratory. The a/LCI technique has great potential as an early-cancer diagnostic that will be realized by implementing and applying robust a/LCI instruments for both clinical and laboratory investigations of cells and tissues.2. The validation and development of Fourier-domain LCI, a novel method for probing sub-surface structure in biological tissues using optical spectroscopy. This approach is a simpler optical scheme than a/LCI but to date has only been demonstrated on tissue phantoms. Further experiments will help to evaluate its potential capabilities.3. The development of a novel microscopy scheme based on using an actively stabilized interferometer to measure the phase of scattered light for cell biology applications. This technique will enable precision measurements of the biophysical properties of cells. The education plan will incorporate photonics into the biomedical engineering curriculum by developing a new Biophotonics course and new teaching materials. The specific activities include development of a modern microscopy course, incorporation of Biophotonics into existing BME courses and development of independent Biophotonics projects for students ranging from high school to the graduate level. The activities will take advantage of a new Biophotonics teaching laboratory that will house state of the art equipment for instructional use. The education program also will benefit from the help of the outreach program at Duke University that will enable introduction of Biophotonics to high school students, through independent projects and to underrepresented groups by involving visiting students in research projects in the principal investigator's laboratory.

0348204Wax此职业发展计划的重点是利用光子学领域的光学知识进行生物医学工程应用。 该研究计划的目标是开发低相干干涉测量（LCI）的新应用，用于检测细胞和组织散射的光，作为一种具有亚波长灵敏度的确定其结构和功能特征的方法。光学技术具有非侵入性、非电离性的优点，并且易于通过简单、经济高效的方式实施，同时避免了对固定剂和染色剂及其相关伪影的需要。该研究计划基于三项活动： 1. 进一步开发角度分辨 LCI (a/LCI)，这是一种原位测量细胞形态测量的方法，其功效已在实验室得到验证。 a/LCI 技术作为早期癌症诊断具有巨大的潜力，这将通过在细胞和组织的临床和实验室研究中实施和应用强大的 a/LCI 仪器来实现。2． 傅里叶域 LCI 的验证和开发，这是一种利用光谱学探测生物组织次表面结构的新方法。这种方法是比 a/LCI 更简单的光学方案，但迄今为止仅在组织模型上进行了演示。进一步的实验将有助于评估其潜在能力。3． 基于使用主动稳定干涉仪来测量细胞生物学应用的散射光相位的新型显微镜方案的开发。该技术将能够精确测量细胞的生物物理特性。该教育计划将通过开发新的生物光子学课程和新的教材，将光子学纳入生物医学工程课程。具体活动包括开发现代显微镜课程、将生物光子学纳入现有的 BME 课程以及为从高中到研究生水平的学生开发独立的生物光子学项目。这些活动将利用一个新的生物光子学教学实验室，该实验室将配备最先进的教学设备。该教育计划还将受益于杜克大学外展计划的帮助，该计划将通过独立项目向高中生介绍生物光子学，并通过让访问学生参与首席研究员实验室的研究项目向代表性不足的群体介绍生物光子学。