Angle resolved light scattering through a multimode fiber for measuring cell nuclei morphology

通过多模光纤进行角度分辨光散射，用于测量细胞核形态

• 批准号: 2009841
• 负责人: Adam Wax
• 金额: $ 55.57万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2009841&HistoricalAwards=false
• 关键词: Angle; resolved; light; scattering; through

This project will create new methods of using optical fibers for examining tissue for early signs of cancer. Although optical detection of early cancer has been shown, there has yet to be a technology that can be readily used by clinicians. This new method will be simpler and less costly to build than previous approaches and thus will provide a societal benefit by enabling greater use of optical methods for detecting early cancer in the clinic.The proposed project will develop a novel approach for detecting early cancer based on angle resolved light scattering. The unique aspect of this new approach is that rather than measuring scattering at different angles at the tissue surface, instead a single, multimode fiber will be used to collect all of the angular scattering and then computational methods will be used to reconstruct the angular distribution. The approach will permit simplified measurements and analysis by using knowledgeof the distribution of scattering from cell nuclei, specifically that it forms ringed patterns. A key proposed advance of this work will be to enable use of the fiber even when it is moved, for example during an endoscopy procedure, by leveraging the simplified measurements and introducing a real time calibration method. The approach will be demonstrated by measuring enlarged nuclei in cultured cancer cells.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将创建使用光纤检查组织以发现癌症早期迹象的新方法。虽然早期癌症的光学检测已经被证明，但还没有一种技术可以方便地被临床医生使用。这种新方法将比以前的方法更简单、成本更低，因此将通过在临床上更多地使用光学方法来检测早期癌症来提供社会效益。该项目将开发一种基于光学方法检测早期癌症的新方法。角分辨光散射。这种新方法的独特之处在于，不是测量组织表面不同角度的散射，而是使用单个多模光纤来收集所有角度散射，然后使用计算方法来重建角度分布。 该方法将通过利用细胞核散射分布的知识，特别是它形成环形图案，来简化测量和分析。 这项工作的一个关键进展是，通过利用简化的测量并引入实时校准方法，即使在移动时也能使用光纤，例如在内窥镜检查过程中。 该方法将通过测量培养癌细胞中增大的细胞核来证明。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Reconstruction of angle-resolved backscattering through a multimode fiber for cell nuclei and particle size determination.

通过多模光纤重建角度分辨反向散射，用于细胞核和颗粒尺寸测定。

• DOI: 10.1063/5.0011500
• 发表时间: 2020
• 期刊: APL photonics
• 影响因子: 5.6
• 作者: Zhang,Haoran;Steelman,ZacharyA;Ceballos,Silvia;Chu,KengyehK;Wax,Adam
• 通讯作者: Wax,Adam