Acquisition of Equipment by a Group of Emory University Developmental Geneticists for Sorting Multicellular Organisms

埃默里大学发育遗传学家团队购买了用于分选多细胞生物的设备

• 批准号: 0216418
• 负责人: Steven L'Hernault
• 金额: $ 10.7万
• 依托单位: Emory University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0216418&HistoricalAwards=false
• 关键词: Acquisition; Equipment; Group; Emory; University

A grant has been awarded to Dr. Steven W. L'Hernault at Emory University to support the purchase of a COPAS (Complex Object Parametric Analyzer and Sorter) cell sorter instrument. These funds will be used to purchase instrumentation that will be utilized by seven investigators that study either the fruit fly Drosophila melanogaster or the roundworm Caenorhabditis elegans, which are species that are extensively utilized in developmental genetic experiments. Genetic experiments in these species usually employ relatively small numbers of animals because these multi-cellular animals are usually sorted by hand. Consequently, obtaining and analyzing large numbers of mutant animals is frequently impossible, especially if the mutant either dies during its maturation (is lethal) or is sterile. Biochemical approaches require large populations of such mutants and, until recently, this was not possible. However, the newly developed COPAS technology now permits accurate sorting and dispensing of multi-cellular animals based on size and/or fluorescence differences. The COPAS can be used to sort either Drosophila or Caenorhabditis, and this permits two different experimental approaches. It is possible to construct Drosophila and Caenorhabditis strains where a specified chromosome is "marked" by the presence of a fluorescent green signal. Mutants can be sorted away from non-mutant siblings by selecting non-Green individuals, so this machine makes it feasible to purify large numbers of lethal or sterile animals. Secondly, it is possible to rapidly dispense a defined number of individuals of a chosen sex or life cycle stage with ~98% accuracy. This permits evaluating individual animals on a scale that is not possible by manual techniques and allows isolated mutants to be assessed for desired, but rare, events. The relevance of Drosophila and Caenorhabditis to significant human problems is well established; Drosophila is a model for the Mediterranean fruit fly, a significant agricultural pest, and Caenorhabditis is a model for a wide variety of parasitic nematodes that infect man, pets, domestic livestock and agriculturally significant plants. The COPAS technology permits application of innovative biochemical techniques to organisms that have traditionally been used for genetic analyses. Additionally, the COPAS is a single machine, cost-effective way for both Drosophila and Caenorhabditis investigators to gain access to a technology that permits these exciting, new experimental approaches.

已向埃默里大学（Emory University）的史蒂文·W·赫纳尔特（Steven W. L'Hernault）博士授予了赠款，以支持购买COPAS（复杂对象参数分析仪和分落者）细胞分落器仪器。这些资金将用于购买仪器，这些仪器将被七位研究人员使用，这些研究人员研究了果蝇果蝇甲虫类或rount虫秀丽隐杆线虫，这些木星是在发育遗传实验中广泛使用的物种。 这些物种中的基因实验通常使用相对较少的动物，因为这些多细胞动物通常是手工分类的。 因此，经常不可能获得和分析大量突变动物，尤其是如果突变体在成熟（致命）或无菌的情况下死亡。 生化方法需要大量这种突变体的种群，直到最近，这是不可能的。 但是，新开发的COPAS技术现在允许根据大小和/或荧光差异对多细胞动物进行准确的分类和分配。 COPA可用于分类果蝇或核hab炎，这允许两种不同的实验方法。 可以构建果蝇和核虫菌株，其中存在荧光绿色信号“标记”指定的染色体。 突变体可以通过选择非绿色个体来从非突变的兄弟姐妹中分类，因此该机器使净化大量致死或无菌动物的可行性。 其次，可以快速分配〜98％精度的选择的性别或生命周期阶段的定义数量的个体。 这可以通过手动技术不可能评估单个动物，并允许对所需但罕见的事件进行评估孤立的突变体。 果蝇和Caenorhabditis与重大人类问题的相关性已很好。果蝇是地中海果蝇的模型，一种重要的农业害虫，而Caenorhabditis是一种型号的模型，用于感染人类，宠物，家庭牲畜和农业意义的各种寄生虫线虫。 COPAS技术允许将创新的生化技术应用于传统上用于遗传分析的生物体。 此外，对于果蝇和Caenorhabditis研究人员来说，COPAS是一台机器，具有成本效益的方式，可以访问允许这些令人兴奋的新实验方法的技术。