Genetics of Sperm Migration and Gamete Recognition in Caenorhabditis elegans

秀丽隐杆线虫精子迁移和配子识别的遗传学

• 批准号: 9305058
• 负责人: Steven L'Hernault
• 金额: $ 31万
• 依托单位: Emory University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9305058&HistoricalAwards=false
• 关键词: Genetics; Sperm; Migration; Gamete; Recognition

9305058 L'Hernault Motile cells can move toward a target site, where they interact with other cells found at this new location. Sperm migration and subsequent sperm-egg interactions in the nematode Caenorhabditis elegans offers an attractive model system in which to study the genetic control of these phenomena. There are mutations in seven known genes which result in motile spermatozoa that are incapable of fertilization. Mutations in three of these spermatogenesis- defective genes are known to produce cytologically normal sperm that are motile, but defective in sperm migration toward the normal site of fertilization and in sperm-egg interactions at that site. These genes will be subjected to detailed cell biological, genetic, and molecular analyses. Specifically, mutants will be analyzed by electron microscopic and other cell biological techniques to reveal any obvious alterations in either sperm structure or sperm interactions with oocytes. Additional alleles will be recovered and the null phenotype will be defined for these three genes. Alleles that will aid in the molecular cloning of these genes will also be sought. Cosmid transgenic rescue will be employed to localize one of these genes on the available C. elegans physical map and permit its molecular cloning. These studies should reveal how sperm recognize their location in the reproductive tract, move towards oocytes, and subsequently fertilize them. ***

9305058 L'HERNAULT MINIMILE细胞可以向目标部位移动，并与在此新位置发现的其他细胞相互作用。 线虫秀丽隐杆线虫中的精子迁移以及随后的精子 - 蛋之间的相互作用提供了一个有吸引力的模型系统，可以在其中研究这些现象的遗传控制。 七个已知基因中有突变导致运动精子无能力受精。 已知这些精子发生缺陷的基因中的三个突变会产生流动性的细胞学正常精子，但在精子迁移到正常受精部位和该部位的精子 - 卵子相互作用方面有缺陷。 这些基因将进行详细的细胞生物学，遗传和分子分析。 具体而言，将通过电子显微镜和其他细胞生物学技术分析突变体，以揭示精子结构或精子与卵母细胞相互作用的任何明显改变。 将回收其他等位基因，并将为这三个基因定义空表型。 还将寻求有助于这些基因分子克隆的等位基因。 宇宙转基因救援将用于将这些基因之一定位在可用的秀丽隐杆线虫物理图上，并允许其分子克隆。 这些研究应揭示精子如何识别其在生殖道中的位置，朝卵母细胞移动，然后将其施肥。 ***