Acquisition of a Confocal Laser Scanning Microscope

购买共焦激光扫描显微镜

• 批准号: 0215852
• 负责人: Sophia Passy
• 金额: $ 28.76万
• 依托单位: University of Texas at Arlington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-15 至 2006-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0215852&HistoricalAwards=false
• 关键词: Acquisition; Confocal; Laser; Scanning; Microscope

ABSTRACT A grant has been awarded to Dr. Sophia Passy (PI), Drs Grover, Marshall, Wilk, and Nelson (Co-PIs), and Drs Arnott, Burleson, and Smith (Senior Personnel) from the University of Texas at Arlington to purchase a confocal scanning laser microscope. The instrument will be used for a broad range of applications, including subcellular localization of molecules; monitoring ion channel clustering patterns; cytoskeleton visualization; emission fingerprinting, 3D reconstruction, and detection of gradients in biofilms. The unifying theme underlying these diverse research endeavors is the search for a relationship between structure and function in the organic world, which remains one of the most fundamental questions in biology.The equipment will be employed for the study of: 1) biofilm spatio-temporal dynamics as a product of small-scale proximate ecological processes of species dispersal, growth, and competition, and ultimate broad-scale environmental constraints of shear stress and nutrient limitation; 2) spectral fingerprinting of live algal communities developing in the freshwater plankton and benthos for the purpose of developing a new biomonitoring approach; 3) chemical and neural mechanisms that control cardiovascular and ventilatory reflex responses to environmental stimuli in fish; 4) three-dimensional reconstructions of sperm shape and structure through optical sectioning of the sperm cytoskeleton to assess the influence of variation in sperm shape on sperm motility and competition; 5) spinal cord and peripheral nerve regeneration by fabricating tissue engineered scaffolds that deliver drugs, proteins, growth factors, and DNA to support and regulate neuronal regeneration; 6) molecular mechanisms used by bacteria to establish and maintain Mg2+ homeostasis, and the consequences of disrupting these mechanisms, and 7) intracellular pathways involved in the regulation of ion channels and how alterations in the functional state of channels affect cellular functions under conditions of chronic pain and inflammation. This microscope will also provide the basis for the development of a new graduate course in Molecular Cytology and enhance existing courses such as Limnology and Phycology. The Biology Department at the University of Texas at Arlington has well established a specialized microscopy facility, the Center for Electron Microscopy, where generations of researchers and students have received training and explored the wonders of the organic and nonliving micro-cosmos. The addition of a confocal microscope to this facility will open new avenues for novel and interdisciplinary research, which should come as no surprise given that confocal microscope technology only became commercially available in the early 1980s, with its use largely limited to medical studies. Hence, there are still a number of unexplored fields in biology, ranging from physiology and signal transduction to aquatic ecology and evolution, which will vastly benefit from the new approaches that confocal microscopy offers.

摘要已授予Sophia Passy博士（PI），Grover博士，Marshall，Wilk和Nelson（Co-​​Pis），以及来自德克萨斯大学阿灵顿大学的Arnott，Burleson和Smith（高级人员）博士，购买共同扫描激光示例激光镜。该仪器将用于广泛的应用，包括分子的亚细胞定位；监视离子通道聚类模式；细胞骨架可视化；发射指纹识别，3D重建和生物膜中梯度的检测。这些多元化研究努力的统一主题是寻找有机世界中结构与功能之间的关系，这仍然是生物学中最基本的问题之一。该设备将用于研究：1）生物膜时空动力学作为生物膜时空动力学，作为小规模生态范围的生态范围的生态范围，以及宽阔的环境，以及范围的生态范围，以及范围的扩展，以及范​​围的扩展，以及宽广的竞争，及其竞争，竞争，竞争，竞争，竞争，竞争，竞争，竞争，竞争，竞争，竞争，竞争，竞争，竞争，竞争，竞争，以及限制； 2）在淡水浮游生物和Benthos中开发的现场藻类社区的光谱指纹识别，目的是开发一种新的生物监测方法； 3）控制鱼类环境刺激的心血管和通气反射反应的化学和神经机制； 4）精子形状和结构的三维重建通过精子细胞骨架的光学切片，以评估精子形状变异对精子运动和竞争的影响； 5）通过制造提供药物，蛋白质，生长因子和DNA的组织工程脚手架来支持和调节神经元再生，脊髓和周围神经再生； 6）细菌用来建立和维持MG2+稳态的分子机制，以及破坏这些机制的后果，以及7）与调节离子通道调节的细胞内途径以及通道功能状态的变化如何影响慢性疼痛和炎症条件下的细胞功能。该显微镜还将为开发分子细胞学新研究生课程的发展提供基础，并增强现有课程，例如羊水学和植物学。德克萨斯大学阿灵顿分校的生物学系已经建立了一个专门的显微镜设施，即电子显微镜中心，几代研究人员和学生已经接受了培训并探索了有机和非生命的微型cosmos的奇迹。在该设施中增加共聚焦显微镜将为新颖和跨学科研究开辟新的途径，鉴于共焦显微镜技术仅在1980年代初在商业上销售，这在很大程度上限于医学研究不足为奇。因此，生物学中仍然有许多未开发的领域，从生理学和信号转导到水生生态学和进化，这将大大受益于共焦显微镜提供的新方法。