MRI: Acquisition of a Zeiss LSM980 Laser Scanning Confocal Microscope with Airyscan 2 for Microscopy Core Facility

MRI：采购蔡司 LSM980 激光扫描共焦显微镜和 Airyscan 2 用于显微镜核心设施

• 批准号: 2117484
• 负责人: Edward Bonder
• 金额: $ 55.45万
• 依托单位: Rutgers University Newark
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2117484&HistoricalAwards=false
• 关键词: MRI; Acquisition; Zeiss; LSM980; Laser

An award is made to Rutgers University – Newark to acquire a Zeiss LSM980 Laser Scanning Confocal Microscope with Airyscan 2. The Zeiss LSM 980 will be integrated into the Advanced Imaging Core Facility (AICF), of the Department of Biological Sciences (DBS), a core university facility that serves Rutgers and institutions of higher education in Newark. Installation of the microscope provides critically needed instrumentation that will have an unquestionable positive impact at Rutgers-Newark as an anchor institution with broad mission to improve and advance STEM education, training, and outreach. Acquisition of the Zeiss LSM 980 will initiate improvement in the undergraduate curriculum in Biology by stimulating development of a six-week long biological imaging laboratory series in a core biology course that enrolls 600 students annually. Using in-person and virtual teaching/learning strategies, students will be able to have an exhilarating first-hand experience at multi-dimensional imaging. DBS will add hands-on laser confocal imaging to a capstone upper division laboratory course in microscopy. To reach a broader undergraduate and graduate student audience, the leadership team will work with NSF-LSAMP, NIH-G-RISE, and Rutgers-NASA Space Grant Consortium programs at Rutgers-Newark to introduce multi-dimensional imaging using the Zeiss LSM 980. It is anticipated that this approach will encourage increased undergraduate participation in research, particularly URM students, and encourage pursuit of STEM careers. STEM faculty at Rutgers-Newark have a long-standing tradition of partnering with American Chemical Society Project SEED and Newark Public Schools to provide urban high school students with hands-on laboratory experience. Microscopy is often a key part of that experience. The leadership team is invested in bringing the wonder of fluorescence imaging to local high school students through the 4-week summer immersion program RUN-IMAGE and the academic year program Aim High NPS-NorthStar Academy. Each of these programs will benefit from both hands-on and virtual imaging relying upon the Zeiss LSM 980 and other microscopes in the AICF. The addition of the Zeiss LSM 980 to the AICF will have societal impacts on multiple fronts. Introducing advanced imaging to students at all levels will improve science literacy and build an understanding of the process of scientific discovery and application. This will have a future positive impact as US citizens are asked to make important societal decisions dependent upon an understanding of science. Further, the Zeiss LSM 980 will strengthen the ability of Rutgers-Newark to hire and retain top-shelf faculty whose research and teaching skills will add to education, training, and outreach at Rutgers-Newark. The LSM 980 will provide new faculty in the biological science with an essential modern-day research instrument that is vital for success in obtaining extramural grant funding and in publishing peer-reviewed work.The Zeiss LSM 980 with Airyscan 2 provides a state-of-the-art microscopy workstation that provides multi-dimensional fluorescence imaging as well as super-resolution research capacity. The microscope will support fundamental research examining the mechanisms of cell signaling and membrane-protein sorting in neural, intestinal epithelial, and bacterial cells. The Zeiss LSM 980 will enable researchers will use novel fluorescent-reporter proteins in conjunction with innovative genetic animal and in vitro cell/organoid models to follow the dynamics and regulatory function of small GTPases and transcription factors in regulating cell and tissue differentiation and maintenance. Super-resolution fluorescence imaging and photo-manipulation will be used to discover novel protein-membrane and protein-protein interactions in the assembly of macromolecular complexes that regulate lipid transport, assembly of cell surface receptors, and intracellular sorting of signaling complexes in bacterial membranes, during myelin formation in peripheral nervous system, and embryonic development in mammalian central. The Zeiss LSM980 will be used in state-of-the-art optogenetic experimentation on the important role of cytoskeleton-chromatin interactions on gene expression and epigenetics. Additionally, the microscope will be used to provide multi-dimensional fluorescence imaging of cell signaling and remodeling pathways activated in response to tissue and cell injury from trauma including, impact, stretch, radiation, and chemical insult. All the research projects are fully integrated with STEM education/training of high school, undergraduate, graduate students and postdoctoral students serving as the foundation for improved STEM literacy and future leadership.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

向罗格斯大学（Rutgers University）颁发了奖项 - 纽瓦克（Newark）获得Zeiss LSM980激光扫描共聚焦显微镜与Airyscan 2。显微镜的安装提供了迫切需要的仪器，该工具将对Rutgers-Newark毫无疑问地积极影响，作为一家主持机构，具有广泛的使命，以改善和推进STEM教育，培训和外展。 Zeiss LSM 980的获取将通过在核心生物学课程中刺激一个为期六周的生物学成像实验室系列的生物学的本科课程来提高生物学的本科课程，该课程每年招收600名学生。使用面对面和虚拟教学/学习策略，学生将能够在多维成像方面具有令人振奋的第一手经验。 DBS将在显微镜中的顶峰上层实验室课程中添加动手激光共聚焦成像。为了吸引更广泛的本科和研究生听众，领导团队将与NSF-LSAMP，NIH-G-RISE和RUTGERS-NASA NASA SPACE GRANT CONSTIUM联盟计划合作，以介绍多维成像，以引入Zeiss LSM 980，预计这种方法将鼓励人们培训不足的学生，并促进学生的培训，并在研究中促成研究培训，并在研究中，以及您的研究培训，以及您的研究培训，以及您的研究培训，以及您的培训培训，以及您的研究培训，并鼓励您在研究中，并在研究中会增加您的培训，并且您的参与培训会增加您的培训，并且在研究过程中会鼓励您的参与者，并鼓励您在研究中，并鼓励您的参与者，并鼓励您的参与者，并鼓励您的参与培训。 Rutgers-Newark的STEM教师具有与美国化学学会项目种子和纽瓦克公立学校合作的悠久传统，为城市高中生提供动手实验室的经验。显微镜通常是这种经验的关键部分。通过为期4周的夏季沉浸式计划Run-Image和学年计划AIM NPS-NORTSTAR Academy，领导团队投资将荧光成像的奇迹带给当地高中生。这些程序中的每一个都将受益于依靠Zeiss LSM 980和AICF中其他显微镜的动手和虚拟成像。在AICF中添加Zeiss LSM 980将对多个方面产生社会影响。向各个级别的学生介绍先进的成像将提高科学素养，并建立对科学发现和应用过程的理解。这将产生未来的积极影响，因为要求美国公民做出重要的社会决策，取决于对科学的理解。此外，Zeiss LSM 980将增强Rutgers-Newark雇用和保留顶级教师的能力，他们的研究和教学技能将增加Rutgers-Newark的教育，培训和外展。 LSM 980将提供一项重要的现代研究工具，为生物学科学提供新的教师，该工具对于获得校园外赠款资金和发布同行评审工作至关重要。Zeiss LSM 980与Airyscan 2提供了最先进的显微镜工作站，可提供一项可提供多维流量研究的最高效率。显微镜将支持研究神经，肠上皮和细菌细胞中细胞信号传导和膜 - 蛋白质的机制的基础研究。 Zeiss LSM 980将使研究人员能够与创新的遗传动物和体外细胞/器官模型结合使用新型的荧光蛋白质蛋白，以遵循小GTPase的动态和调节功能，以及调节细胞分化和组织分化和维持的小GTPase和转录因子的动态和调节功能。超分辨率的荧光成像和光学操作将用于在大分子分子复合物组装中发现新型蛋白质膜和蛋白质 - 蛋白质相互作用，这些复合物调节脂质转运，细胞表面受体的组装以及在细菌膜中的信号复合物的细胞内分类，在细菌膜中，在骨髓蛋白膜中的形成过程中，并胚胎神经膜上的发育和胚胎的胚胎形成。 Zeiss LSM980将用于最先进的光遗传学实验，讲述细胞骨架 - 染色质相互作用对基因表达和表观遗传学的重要作用。此外，显微镜将用于提供细胞信号传导和重塑途径的多维荧光成像，这些途径响应于组织和受伤的细胞损伤，包括，撞击，拉伸，辐射和化学侮辱。所有研究项目均与高中，本科，研究生和博士后学生的STEM教育/培训完全融合在一起，这些学生是改善STEM识字和未来领导力的基础。这项奖项反映了NSF的法定任务，并被认为是通过基金会的知识分子优点和更广泛的影响审查标准来通过评估来获得的支持。