PECASE: Development of a New Mass Spectrometry-Based Approach for Assaying Protein Stability and Initiation of a High School Science Teacher Outreach Program

PECASE：开发一种新的基于质谱的方法来测定蛋白质稳定性并启动高中科学教师外展计划

• 批准号: 0094224
• 负责人: Michael Fitzgerald
• 金额: $ 53万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-15 至 2006-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0094224&HistoricalAwards=false
• 关键词: PECASE; Development; New; Mass; Spectrometry

AbstractProposal: 0094224 Date: March 18, 2002PI: Michael C. Fitzgerald Institution: Duke University This PECASE award to Professor Michael Fitzgerald of Duke University is supported by the Analytical and Surface Chemistry Program in the Chemistry Division. The primary research objective is to develop a mass spectrometry based method to examine protein stability and folding. The experimental technique of hydrogen-deuterium exchange combined with matrix assisted laser desorption ionization mass spectrometry has several advantages over existing techniques for studying protein folding. The long-range goals of this research are to develop a method to study complex binding interactions between multiple proteins and DNA. The educational component of this program is both innovative and well developed. Dr. Fitzgerald presents a program focused on a summer workshop for high school science teachers. He collaborated with the school district science specialist in the development of the plan, which has also received the support of eight regional principals. This research will impact the fields of mass spectrometry and biochemistry. Compared to established methods, this method is not limited by sample purity and will have greater sensitivity. It is designed to be automated and fast. Future applications of this line of research could impact the study of both disease state and normal human health.

摘要提案：0094224 日期：2002 年 3 月 18 日 负责人：Michael C. Fitzgerald 机构：杜克大学 授予杜克大学 Michael Fitzgerald 教授的 PECASE 奖得到了化学部分析和表面化学项目的支持。 主要研究目标是开发一种基于质谱的方法来检查蛋白质稳定性和折叠。 氢-氘交换实验技术与基质辅助激光解吸电离质谱相结合，与现有的蛋白质折叠研究技术相比具有多种优势。 这项研究的长期目标是开发一种方法来研究多种蛋白质和 DNA 之间复杂的结合相互作用。 该计划的教育部分既创新又完善。 菲茨杰拉德博士提出了一个针对高中科学教师的夏季研讨会的计划。 他与学区科学专家合作制定了该计划，该计划还得到了八位地区校长的支持。这项研究将影响质谱和生物化学领域。 与已有的方法相比，该方法不受样品纯度的限制，并且具有更高的灵敏度。 它被设计为自动化且快速。这一系列研究的未来应用可能会影响疾病状态和正常人类健康的研究。