Biomineralization and self-assembly of genetically modifiable nanofibers to build bone tissue engineering scaffolds

基因修饰纳米纤维的生物矿化和自组装构建骨组织工程支架

• 批准号: 0854414
• 负责人: Chuanbin Mao
• 金额: $ 38.55万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0854414&HistoricalAwards=false
• 关键词: Biomineralization; self; assembly; genetically; modifiable

This award is funded under the American Recovery and Reinvestment Act of 2009 Public Law 111-5).0854414Mao Intellectual merit This project will advance the fields of bone biomineralization and tissue engineering while promoting teaching, training and learning. A peptide that can specifically not only bind to but also nucleate hydroxylapatite (HAP) nanocrystals will be identified by phage display. Phage that double-displays this binding/nucleating peptide and the cell-adhesion peptide on its surface can be site-specifically biomineralized and selfassemble into a scaffold, which mimics the chemical composition and hierarchical architecture of bone extracellular matrix (ECM) and thus is expected to have biological functions of natural bone ECM. The resultant ECM-like scaffold can support the proliferation and differentiation of mesenchymal stem cells (MSCs) to form bone in vitro. Broader impacts This project will have impacts on materials science, chemistry, biology, bioengineering and medicine. It shows that phage is a good platform for studying the mechanism of biomineralization and can be applied to develop scaffolds for the engineering of other tissues. It will also be integrated into outreach and educational activities and have many benefits. It will facilitate the development of a new curriculum on bionanotechnology at Oklahoma University to educate a new generation of workforce in the field of bionanotechnology. It will allow bioengineering PhD and undergraduate students to be trained to satisfy the urgent need of multidisciplinary talents in bionanotechnology. Community college students will be motivated to pursue higher education. A Native American Nanotechnology Outreach (NANO) program in Oklahoma that has recently been initiated by the PI will be expanded and solidified by partnering with Native American students, high school educators and the Native American Research Center for Health (NARCH) program in Oklahoma. This program will help Oklahoma Native Americans become interested in scientific careers and competitive in job markets. Public awareness of bionanotechnology will be increased in Oklahoma statewide by partnering with the Oklahoma Nanotechnology Initiative, and more Oklahomans will be Speaking of Nano

该奖项是根据2009年《美国复苏与重新投资法》的资助。111-5）.085414MAO知识优点该项目将在促进教学，培训和学习的同时推动骨生物矿化和组织工程领域的领域。不仅可以特异性结合，而且还可以通过噬菌体显示识别核羟基磷灰石（HAP）纳米晶体的肽。将这种结合/成核肽和表面上的细胞粘附肽进行双向斑点的噬菌体可以特定于生物矿化和自我组件成脚手架，并模拟骨外细胞外基质（ECM）的化学组成和层次结构，因此预计具有生物学功能的生物学功能。所得的类似ECM的支架可以支持间充质干细胞（MSC）的增殖和分化，以在体外形成骨骼。更广泛的影响该项目将对材料科学，化学，生物学，生物工程和医学产生影响。它表明噬菌体是研究生物矿化机制的好平台，可以应用于开发其他组织工程的支架。它也将融入外展和教育活动中，并有很多好处。它将促进俄克拉荷马大学的Bionanotechnology新课程的开发，以在Bionanotechnology领域教育新一代的劳动力。它将允许对生物工程博士学位和本科生进行培训，以满足迫切需要Bionanotechnology中多学科才能的需求。 社区大学生将有动力接受高等教育。俄克拉荷马州的一项美国原住民纳米技术外展计划（NANO）计划最近由PI发起，将通过与俄克拉荷马州原住民学生，高中教育者和美国原住民研究中心（NARCH）计划合作扩大和巩固。该计划将帮助俄克拉荷马州原住民对科学职业感兴趣，并在就业市场上具有竞争力。通过与俄克拉荷马州纳米技术计划合作，全州俄克拉荷马州的公众对Bionanotechnology的认识将提高，而更多的俄克拉荷马人将谈论Nano