Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia

合作研究：产溶剂梭菌的遗传分析和代谢工程

• 批准号: 9905669
• 负责人: Eleftherios Papoutsakis
• 金额: $ 81.83万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9905669&HistoricalAwards=false
• 关键词: Collaborative; Research; Genetic; Analysis; Metabolic

The objective of the project is to investigate the metabolic engineering of solventogenic Clostridia using modern molecular biology and genetic techniques. The primary goal of this interdisciplinary proposal is to genetically alter the metabolism of Clostridium acetobutylicum. The proposal describes four areas of activity; (1) a detailed examination of the regulation of the sol locus genes through a putative repressor, SolR; (2) a molecular analysis of the function of sporOA and of ORF (orfY) gene products, both of which are likely to be important in the expression of the sol locus genes; (3) an analysis of patterns of gene expression using cDNA arrays; and (4) the further development of antisense RNA as a metabolic engineering tool.

该项目的目的是使用现代分子生物学和遗传技术研究溶剂封闭的代谢工程。 该跨学科建议的主要目标是在遗传上改变乙梭状芽胞杆菌的代谢。 该提案描述了四个活动领域； （1）通过推定的阻遏物SOLR对SOL基因基因的调节进行详细检查； （2）对Sporoa和ORF（ORFY）基因产物功能的分子分析，它们在SOL基因座基因的表达中可能很重要； （3）使用cDNA阵列对基因表达的模式进行分析； （4）反义RNA作为代谢工程工具的进一步开发。