ME: Metabolic Engineering of Solvent Tolerance in Anaerobic Bacteria

ME：厌氧细菌溶剂耐受性的代谢工程

• 批准号: 9911231
• 负责人: Eleftherios Papoutsakis
• 金额: $ 34.42万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9911231&HistoricalAwards=false
• 关键词: ME; Metabolic; Engineering; Solvent; Tolerance

The objective of this project is to understand the molecular basis of solvent tolerance in Clostridium acetobutylicum, with the purpose of using this information to obtain strains that produce higher concentrations of solvents. The research is organized around two hypotheses: (1) that the ability to produce high concentrations of solvent (butanol), and also butanol tolerance, is enhanced by increased protein stability in strains that overexpress heat-shock proteins; and (2) that butanol tolerance is enhanced by expression of native solvent/drug resistance genes, e. g. proteins of the multidrug efflux family. This project is being jointly funded by two programs at NSF and by the Environmental Protection Agency (EPA).

该项目的目的是了解丙酮丁醇梭菌溶剂耐受性的分子基础，目的是利用这些信息获得产生更高浓度溶剂的菌株。 该研究围绕两个假设进行：(1) 过度表达热休克蛋白的菌株中蛋白质稳定性的增加可增强产生高浓度溶剂（丁醇）的能力以及丁醇耐受性； (2)丁醇耐受性通过天然溶剂/药物抗性基因的表达而增强，例如。 g。多药外排家族的蛋白质。 该项目由 NSF 和美国环境保护局 (EPA) 的两个项目联合资助。