SBIR Phase II: Seed Treatment with Fungal and Bacterial Antagonists for Reducing Corn Damping Off and Root Rot Caused by Species of Pythium and Fusarium

SBIR 第二阶段：用真菌和细菌拮抗剂进行种子处理，以减少腐霉和镰刀菌引起的玉米猝倒和根腐病

• 批准号: 9901629
• 负责人: Tom Johnson
• 金额: $ 39.79万
• 依托单位: TJ Technologies, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9901629&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Seed; Treatment

The disease pathogens of Pythium and Fusarium cause significant losses to corn producers when weather conditions are conducive to the plant pathogens and the seed is not pretreated with a protective fungicide seed treatment. Chemical seed treatments are commercially available to control the effects of these pathogens on seedlings. However, no effective treatment is commercially available to control the root and stalk rots that these pathogens cause in later growth stages of the plant. A combination fungal/bacterial antagonist has been identified that may effectively control these pathogens in the early plant growth stages with continued protection thoughout the growing season. This Small Business Innovation Research (SBIR) Phase II project is directed toward: (1) identifying additonal bacterial antagonists; (2) scaling up of biocontrol fermentation processes; (3) establishing large scale procedures for downstream processing, packaging, and storage; (4) evaluating biocontrol agents in field trials; (5) assessing the stability of the biocontrol agents; and, (6) assessing root colonization of the biocontrol agents. Phase III commercialization will involve EPA approval and marketing of the tested product. This project is designed to develop commercially viable biological disease control agents for corn. Because the product is expected to effectively provide season long disease control, and the present chemical fungicides do not, it is anticipated that hybrid seed companies would readily accept an alternative product that is more effective. The US market potential for this product is estimated to be in excess of 150 million dollars.

当天气条件有利于植物病原体生长且种子未采用保护性杀菌剂种子处理剂进行预处理时，腐霉属和镰刀菌病害病原体会给玉米生产者造成重大损失。 化学种子处理在商业上可用于控制这些病原体对幼苗的影响。 然而，商业上没有有效的治疗方法来控制这些病原体在植物后期生长阶段引起的根腐病和茎腐病。 已经鉴定出一种真菌/细菌组合拮抗剂，可以在植物生长早期有效控制这些病原体，并在整个生长季节提供持续保护。 该小企业创新研究 (SBIR) 第二阶段项目旨在：(1) 识别其他细菌拮抗剂； (2)生物防治发酵工艺的规模化； (3) 建立大规模的下游加工、包装和储存程序； (4) 评价生物防治剂的现场试验； (5)评价生防剂的稳定性； (6) 评估生物防治剂的根部定殖。 第三阶段商业化将涉及 EPA 批准和测试产品的营销。 该项目旨在开发商业上可行的玉米生物病害防治剂。 由于该产品预计能够有效地提供整个季节的疾病控制，而目前的化学杀菌剂则不能，因此预计杂交种子公司将很容易接受更有效的替代产品。该产品的美国市场潜力估计超过1.5亿美元。