POWRE: A Plant Cell Suspension/Plant Viral System for Expression of Heterologous Proteins

POWRE：用于表达异源蛋白的植物细胞悬浮液/植物病毒系统

• 批准号: 9870453
• 负责人: Karen McDonald
• 金额: $ 7.5万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-15 至 2001-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9870453&HistoricalAwards=false
• 关键词: POWRE; Plant; Cell; Suspension; Viral

9870453 McDonald Dr. McDonald wishes to use a POWRE award to initiate a new area of research that will aid her in establishing an independent research activity. The new area involves viral transfection of genes to plant cell suspension cultures. Tobacco mosaic virus will be used to infect tobacco, tomato, and other plant cells. Green fluorescent protein will be used to mark successful transfection. The initial experiments will be run in test tubes and shake flasks and will be scalled-up to a 5L bioreactor if successful. Dr. McDonald will develop new course material on plant cell culture and plant virus transfer of genes. A case study on protein production and isolation from plants and plant cells will be developed for a senior chemical engineering design course. ***

9870453 McDonald McDonald 博士希望利用 POWRE 奖启动一个新的研究领域，以帮助她建立独立的研究活动。 新领域涉及将基因病毒转染到植物细胞悬浮培养物中。 烟草花叶病毒将用于感染烟草、番茄和其他植物细胞。 绿色荧光蛋白将用于标记转染成功。 最初的实验将在试管和摇瓶中进行，如果成功，将被转移到 5L 生物反应器中。 麦克唐纳博士将开发有关植物细胞培养和植物病毒基因转移的新课程材料。 将为高级化学工程设计课程开发关于植物和植物细胞中蛋白质生产和分离的案例研究。 ***