Rapid cell-to-cell and plant-to-plant responses to abiotic stress

对非生物胁迫的快速细胞间和植物间反应

• 批准号: 2343815
• 负责人: Ron Mittler
• 金额: $ 100.87万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-04-01 至 2028-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2343815&HistoricalAwards=false
• 关键词: Rapid; cell; plant; responses; abiotic

Plants play a fundamental role in sustaining life on Earth, converting solar energy into sugars. To achieve optimal productivity, the different parts of the plant, and the plant as a whole, must rapidly acclimate to fluctuating changes in environmental conditions (e.g., changes in light intensity, temperature, humidity, the presence of pathogens, etc.). Because in nature, or under field conditions, not all parts of the plant are simultaneously subjected to the same environmental conditions, plants evolved the ability to rapidly transmit cell-to-cell signals from one part to another, optimizing their overall photosynthetic activity, growth, and productivity. This ability is termed ‘systemic signaling’. A novel live imaging method to detect rapid systemic signals within and between different parts of the same plant (i.e., cell-to-cell) and/or between different plants living in a community (i.e., plant-to-plant) was recently developed. This new method will be used to study different key regulators of rapid systemic signaling within and between different plants and characterize the molecular mechanisms that integrate them. Findings from this research will enable the development of future crop plants with enhanced resilience to global warming, preventing yield losses that are estimated at billions of dollars annually to the US economy. In addition, this research will be disseminated to the public through YouTube videos, radio programs at a local public radio station, outreach activities at the University of Missouri farm, as well as mentored research experiences for undergraduate and high school students.Stress-induced systemic signaling and systemic acquired acclimation play a pivotal role in plant resilience to abiotic stress. A novel imaging method that enables the measuring of rapid whole-plant systemic signals (reactive oxygen species, redox, calcium, and electric) in living plants grown in soil was recently developed. This method was used in previous studies to identify several important regulators of rapid systemic cell-to-cell signaling in plants, as well as to discover aboveground plant-to-plant systemic signaling. However, the mode of integration and hierarchy of systemic cell-to-cell and plant-to-plant signals is currently unknown. Using the newly developed imaging method, the hierarchy, mode of integration, and tissue specificity of different rapid systemic cell-to-cell and plant-to-plant signals triggered by a local application of excess light stress or wounding will be determined. In addition, the type of information being transferred during plant-to-plant signaling in response to excess light stress or wounding, and the protein complexes at the plasma membrane that integrate systemic calcium, reactive oxygen species, and redox signaling will be identified and studied. Results obtained from this study could lead to the development of novel approaches to enhance the resilience of crops to different stresses associated with global warming, as well as to the identification of key multiprotein complexes at the plasma membrane that link reactive oxygen species, redox, and calcium signaling.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物在维持地球上的生命，将太阳能转化为糖中起着基本作用。为了达到最佳的生产率，植物的不同部分以及整个植物必须迅速适应环境条件的变化（例如，光强度，温度，湿度，湿度，病原体等的存在的变化）。因为在本质上或在田间条件下，并非植物的所有部分都受到相同的环境条件的约束，所以植物可以将快速传输细胞对细胞信号从一个部分传播到另一部分，从而优化其整体光合作用活性，生长和生产率。此功能称为“系统性信号”。最近开发了一种新型的实时成像方法，用于检测同一植物（即细胞到细胞）和/或居住在社区中（即植物到植物）之间的不同部分（即细胞到细胞）之间的快速全身信号。这种新方法将用于研究不同植物内部和不同植物之间快速全身信号传导的不同关键调节剂，并表征整合它们的分子机制。这项研究的结果将使未来的作物植物的发展具有增强对全球变暖的韧性，从而防止了每年为美国经济估计数十亿美元的收益损失。此外，这项研究将通过YouTube视频，当地公共广播电台的广播节目，密苏里大学农场的宣传活动以及针对本科生和高中生的指导研究经验将其传播给公众。诱发的系统性信号传导和系统性获得的适应能力在植物弹性中对植物韧性起着至关重要的作用。最近开发了一种新型的全植物全身信号（活性氧，氧化还原，钙和电气）的新型成像方法，最近开发了土壤中生长的生物。在先前的研究中使用了该方法来识别植物中快速全身细胞 - 细胞信号传导的几个重要调节剂，并发现地上植物到植物的全身信号传导。但是，目前尚不清楚全身细胞对细胞和植物对植物信号的集成和层次结构的模式。使用新开发的成像方法，将确定不同快速的全身细胞到细胞和植物对植物信号的层次结构，整合模式以及由局部应用过多的光应力或获胜触发的植物对植物信号的特异性。此外，将在植物到植物信号传导过程中转移的信息类型，以响应过量的光应力或胜利，以及将整合全身性钙，活性氧和氧化还原信号传导整合的质膜上的蛋白质复合物。从这项研究获得的结果可能导致开发新的方法，以增强农作物对与全球变暖相关的不同压力的弹性，以及在质膜上鉴定关键多蛋白络合物的鉴定，这些质膜将活性氧，氧化还原和钙信号联系起来，这些奖项通过NSF的法定宣传和良好的支持，均具有良好的影响。 标准。