A Novel Cell Line and Improved Plant Cell Culture Strategies for the Production of Ribosome Inactivating Proteins

用于生产核糖体失活蛋白的新型细胞系和改进的植物细胞培养策略

• 批准号: 9407177
• 负责人: Karen McDonald
• 金额: $ 25.4万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-15 至 1997-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9407177&HistoricalAwards=false
• 关键词: Novel; Cell; Line; Improved; Plant

9407177 McDonald This project is to advance and broaden our knowledge in the areas of plant cell biotechnology, ribosome inactivating proteins (RIPs), and plant cell bioprocess engineering. This project has three specific goals, which include: (1) characterization of the transformation of plant cell cultures by the plant pathogen Agrobacterium rhizogenes; (2) quantification of cell growth and RIP production in suspension culture; and (3) purification and characterization of proteins obtained from the cell cultures. RIPs are naturally occurring plant proteins thought to be ubiquitous in the plant kingdom, and are currently receiving considerable attention for their antiviral, antitumor and abortifacient properties. They are also being considered for their potential use in immunotoxin preparation and for their ability to confer resistance to plant pathogens. ***

9407177 McDonald 该项目旨在推进和拓宽我们在植物细胞生物技术、核糖体失活蛋白 (RIP) 和植物细胞生物过程工程领域的知识。 该项目有三个具体目标，其中包括：（1）植物病原体发根农杆菌对植物细胞培养物转化的表征； (2)悬浮培养中细胞生长和RIP产生的定量； (3)从细胞培养物中获得的蛋白质的纯化和表征。 RIP 是天然存在的植物蛋白，被认为在植物界中普遍存在，目前因其抗病毒、抗肿瘤和堕胎特性而受到广泛关注。 它们还因其在免疫毒素制备中的潜在用途以及赋予植物病原体抗性的能力而被考虑。 ***