Morphogenesis in Streptomyces

链霉菌的形态发生

• 批准号: 9722960
• 负责人: Janet Westpheling
• 金额: $ 33万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9722960&HistoricalAwards=false
• 关键词: Morphogenesis; Streptomyces; Morphogenesis; Streptomyces

Westpheling 9722960 The primary long-term objective of this study is to understand the regulatory interconnection between carbon source catabolite repression, activation of antibiotic biosynthesis and morphological development in the Streptomyces bacteria. The general approach of this project has been to use catabolite controlled genes involved in complex carbohydrate utilization as tools to probe the connection between carbon utilization and morphogenesis. Using this approach, a new class of mutants was identified that are at once defective in catabolite control and morphogenesis, and also overproduce antibiotics. The most pleiotropic of the known morphological mutants of Streptomyces, bldB, was shown during the course of previous work to be pleiotropically defective in the regulation of carbon utilization. The bldB gene product is required for catabolite repression, the initiation of morphogenesis and antibiotic biosynthesis in S. coelicolor, and the study of how this gene functions in the interconnection of these processes is a major focus of the current project. To facilitate the isolation, cloning, and characterization of new mutants, transposon mutagenesis and generalized transduction methods in S. venezuelae are being developed to be used in combination with S. coelicolor for genetic analysis.

Westpheling 9722960这项研究的主要长期目标是了解碳源分解代谢物抑制，抗生素生物合成的激活和链霉菌细菌形态学发展之间的调节互连。 该项目的一般方法是使用涉及复杂碳水化合物利用的分解代谢产物控制的基因作为探测碳利用和形态发生之间联系的工具。 使用这种方法，确定了一种新的突变体，这些突变体在分解代谢物控制和形态发生方面立即有缺陷，并且也过量产生抗生素。 在先前的工作过程中显示了已知的链霉菌形态突变体BLDB的最多效，在调节碳利用方面是多效性的。 BLDB基因产物是分解代谢产物抑制，形态发生和抗生素生物合成中的启动所必需的，并且研​​究该基因在这些过程的互连中如何发挥作用是当前项目的主要重点。 为了促进新突变体的隔离，克隆和表征，委内瑞拉链球菌中的转座子诱变和广义转导方法已被开发为与Coelicolor结合使用，用于遗传分析。